02504nas a2200241 4500008004100000245010300041210006900144260001500213300001200228490000700240520177100247653003602018653001502054653001502069653001702084653002602101100000502127700001602132700002502148700003402173700001902207856003602226 2021 eng d00aImmunostimulatory Activities of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) in White Male Mice0 aImmunostimulatory Activities of Pegagan Embun Hydrocotyle sibtho cMarch 2021 a368-3750 v133 a

Introduction: Pegagan embun (Hydrocotyle sibthorpioides L.) has many pharmacological activies, such as improving the immune system. Aim: This research aims to study the immunomodulatory effect of Pegagan embun herbs ethanol extract (Hydrocotyle sibthorpioides L.) determined by phagocytic activity and capacity of macrophages, total and percentage of leukocytes. Methods: 25 male white mice were divided into 5 equal groups. Negative control group were given Na CMC 0.5%, the extract treated group were given Pegagan embun ethanol extract at doses of 10, 50, 200 mg/kgbw, and the positive control group was given Stimuno 50 mg/kgbw orally for 7 days. On the 8th day, the total and percentage of leukocytes were counted through blood sample taken intravenously. The mice were then induced with Staphylococcus aureus suspension. After one hour, the peritoneal fluids was taken to determine the macrophages activity and capacity. The macrophages phagocytic activity and capacity, total and percentage of leukocytes were analyzed by One-Way Anova followed by Duncan Multiple Range Test (p<0.05). Results: The results show significant increase of concentration (p<0.05) towards macrophages phagocytic activity and capacity, and total leukocytes count. Percentage of leukocytes show that lymphocytes increase significantly (p<0.05), meanwhile neutrophils segments decrease significantly (p<0.05). Conclusion: It can be concluded that ethanol extract of Pegagan embun herb at doses of 10, 50, 200 mg/kgbw shows immunostimulatory activity.

10aHydrocotyle sibthorpioides Lam.10aLeukocytes10aMacrophage10aPhagocytosis10aStaphylococcus aureus1 a1 aAldi, Yufri1 aDillasamola, Dwisari1 aLarakhansa, Yolanda, Alfiyyah1 aBadriyya, Elsa uhttps://phcogj.com/article/1371