02087nas a2200229 4500008004100000245010900041210006900150260001500219300001200234490000700246520138500253653002001638653001401658653001401672653001601686653001901702100002301721700002401744700002601768700002701794856003601821 2021 eng d00aErgosterol Isolated from Agaricus blazei Murill N-Hexane Extracts as Potential Anticancer MCF-7 Activity0 aErgosterol Isolated from Agaricus blazei Murill NHexane Extracts cMarch 2021 a418-4260 v133 a

Extracts and some of the Agaricus blazrei Murill isolates have potential anticancer. Ergosterol isolate from Amaouroderma rude can also inhibit the growth of MDA-MB-231 cancer cells through apoptotic pathways by increasing FOXO3 expression, while its potency against MCF-7 cells has not been reported. The purpose of this study was to isolate, determine the structure, determine the anticancer activity of MCF-7 cells, and the isolate mechanism by apoptosis from one of isolates the n-hexane A.blazei Murill extracts. This research method includes the isolation of compounds from A.blazei Murill extract by chromatography method guided using Bioactivity Guided Isolation. The structure elucidation of structure isolates used UV, NMR and MS spectroscopy. Anticancer activity test using the MTT cytotoxic test. Eludation of UV, NMR and MS structures showed a ergostrerol. The anticancer activity test showed IC50 values of 43.10 μg/ mL with the strong cytotoxic category. The mechanism of action is to increase apoptosis induction through inhibition of the cell cycle in the G2/ M phase. The conclusion of the isolated compound was ergosterol with an IC50 value of 43.10 μg / mL with an increased apoptosis induction mechanism through inhibition of the cell cycle in the G2/ M phase.

10aAgaricus blazei10aApoptosis10aEgosterol10aMCF-7 cells10aMurill extract1 aMisgiati, Misgiati1 aWidyawaruyanti, Aty1 aRaharjo, Sentot, Joko1 aSukardiman, Sukardiman uhttps://phcogj.com/article/1377