02339nas a2200253 4500008004100000245011300041210006900154260001500223300001200238490000700250520155000257653002801807653001601835653001101851653003901862100002301901700001901924700002201943700001901965700002801984700001602012700002102028856003602049 2021 eng d00aPotential Inhibition by Phaleria macrocarpa Leaves Ethanol Extract on Ki-67 Expression in Distal Colon Mouse0 aPotential Inhibition by Phaleria macrocarpa Leaves Ethanol Extra cMarch 2021 a443-4490 v133 a

Ulcerative colitis (UC) has been an important aspect of an incurable chronic inflammatory disease over the last few decades. To find useful therapies for UC, one of which is herbal therapy, many researches have been conducted. Due to its anti-inflammatory effects, Phaleria macrocarpa (PM), an Indonesian indigenous herb, is considered to be the alternative therapy for UC. Phaleria macrocarpa Leaves Ethanol Extract (PMLEE) is then used in this research to determine its effect on UC by using Ki-67 as a marker of proliferation. PMLEE was created from dry PM content undergoing maceration. The animals were classified into six categories: normal, positive control, negative control and PMLEE group (100, 200, 300 mg/kgBW). PMLEE was then injected for 7 consecutive days into BALB/c mice that were caused by dextran sodium sulphate (DSS). DSS is used for modeling UC in the colon tissue of mice. All mice were terminated and then stained with anti-Ki-67 after their colons were extracted. Subsequently, the stained parts were analyzed with ImageJ based on the color intensity produced by the results of H-score. Based on H-score, PMLEE 300mg and 200mg has significantly decreased the expression of Ki-67 compare to the negative control (p=0.001 and p=0.01). PMLEE also has a tendency to be dose dependent based on the significant difference from PMLEE 300mg and 100mg (p=0.002). It then concludes that PMLEE is related to Ki-67 expression in cells, as it was inversely proportional in this analysis.

10aDextran sodium sulphate10aInflamation10aKi- 6710aMahkota Dewa (Phaleria macrocarpa)1 aKusmardi, Kusmardi1 aWiyarta, Elvan1 aEstuningtyas, Ari1 aSahar, Nurhuda1 aMidoen, Yurnadi, Hanafi1 aTedjo, Aryo1 aPakpahan, Alfred uhttps://phcogj.com/article/1380