02547nas a2200229 4500008004100000245009500041210006900136260001500205300001200220490000700232520187500239653000602114653002602120653001702146653001702163653001002180653002102190653001502211100002202226700003302248856003602281 2021 eng d00aChemical Profile and Antioxidant Properties of Andrographis producta (C. B. Clarke) Gamble0 aChemical Profile and Antioxidant Properties of Andrographis prod cMarch 2021 a475-4850 v133 a

Background: Andrographis producta (Acanthaceae) is endemic to Western Ghats, India, traditionally used by native people for the control of various ailments including intestinal worms, to relieve constipation and also used to eliminate phlegm in women during postpartum. Objective: To investigate the chemical compounds in root, stem and leaves of A. producta and their antioxidant properties. Method: The phytochemical contents were determined using spectrophotometric methods and chemical profiling of root, stem and leaf extracts was carried out using GC-MS. Further, extracts were investigated for their antioxidant capacities using in vitro DPPH radical scavenging and FRAP assay. Results: The total phenolics (163.61 mg GAE/g), flavonoids (35.11 ± 0.53 mg QE/g) and tannins (84.52 ± 0.07 mg TAE/g) were highest in stem compared to leaf and root. Stem was exerted superior antioxidant capacities in both DPPH (EC50 3.58 mg/ml) and FRAP assays (1.742 ± 0.02 OD at 1mg/ml) and were comparable to standards. GC-MS analysis revealed total 89 chemical compounds including phenolics, flavonoids, terpenoids and organic acids. 2-Methoxy-4-vinylphenol (0.70 %), 2,4-ditert- butylphenol (9.74 %), phytol (10.32 %), 5-hydroxy-7,8-dimethoxyflavone (11.42 %), gammasitosterol (8.32 %), salvigenin (12.09 %), solanesol, (2.92 %), and alpha-terpinene (4.58 %) were important bioactive compounds found in significant amount. Conclusion: The present investigations indicate that various parts of A. producta can be explored as good source of antioxidants due to the presence of phenolics and flavonoids. The meticulous assessment of bioactive compounds from A. producta would be great contribution in field of medicine.

10a210a4-Di-tert-butylphenol10aAndrographis10aAntioxidants10aGC-MS10aMethanol extract10aSalvigenin1 aDalawai, Dayanand1 aMurthy, Hosakatte, Niranjana uhttps://phcogj.com/article/1384