02333nas a2200229 4500008004100000245012300041210006900164260001300233300001200246490000700258520164700265653001901912653001401931653002101945653001201966653001301978653001901991100002302010700000502033700002902038856003602067 2021 eng d00aBoth Ethanol and Ethyl Acetate Curcuma Zedoaraia Extract was Capable of Inducing Cells Death in T47D Cell Line Culture0 aBoth Ethanol and Ethyl Acetate Curcuma Zedoaraia Extract was Cap cMay 2021 a737-7430 v133 a

Introduction: Curcuma zedoaria (CZ) has been proven capable of inducing apoptosis in cells cancer. CZ extraction can be performed by ethanol and acetyl acetate as solvent. However, which one of these extracts is superior remains unclear. Objective: This study aimed to investigate the difference potential effect of ethanol and acetyl CZ extract on apoptosis of T47D cell line. Methods: In this study 21 wells were assign into seven groups: control group (T47D); treatment groups consisting of group of ethanol CZ extract 46 (EtZ-46); group of ethanol CZ extract 23 (EtZ-23); group of ethanol CZ extract 11 (EtZ-11); and group of ethyl acetate CZ extract 111 (AcZ-111); group of ethyl acetate CZ extract 55 (AcZ-55); and group of ethyl acetate CZ extract 27 (AcZ-27). In T47D group only loaded with T47D cell line; in treatment groups aside from loaded with T47D cell line culture, also treated with ethanol or acetyl acetate CZ extract respectively. Concentration of T47D cell was 5 x 104 T47D cells line in 100 μl suspension loaded on each well of 21 wells and kept in CO2 incubator overnight. The apoptosis cells were measured after 48 hours post CZ treatment. Results: Post Hoc analysis indicated that the number of apoptosis cells in AcZ-111 was significant higher compared to that of other groups, p<0.05. Conclusion: Acetyl acetate CZ extract treatment with dose 111 μg was capable of inducing apoptosis in T47D cell line superior than that of other groups including ethanol CZ extract.

10aAcetyl acetate10aApoptosis10aCurcuma zedoaria10aEthanol10aNecrosis10aT47D cell line1 aSumarawati, Titiek1 a1 aNasihun, Taufiqurrachman uhttps://phcogj.com/article/1420