03027nas a2200337 4500008004100000245017400041210006900215260001300284300001200297490000700309520193300316653002502249653002902274653001502303653002102318100002902339700003302368700002602401700002302427700002502450700001602475700002002491700001602511700002602527700002302553700002502576700001602601700002002617700001602637856003602653 2021 eng d00aThe Effect of Eurycoma Longifolia Jack (Tongkat Ali) Root Extract on salivary S. Mutans, Lactobacillus and Candida Albicans Isolated from High-Risk Caries Adult Patients0 aEffect of Eurycoma Longifolia Jack Tongkat Ali Root Extract on s cMay 2021 a787-7910 v133 a

Introduction: The roots of E. longifolia jack (E.L.) or Tongkat Ali have been used in traditional medicine as well as supplements and food additives. Many chemical compounds have been detected in extracts of its roots which are believed to be responsible for its medicinal properties. In this study, our objectives were to study the effects of EL root extracts on the growth of Streptococcus Mutans, Lactobacillus and Candida Albicans isolated from saliva of adult patients with high caries risk. Materials and Methods: The ethanolic extract of the root of this plant was tested against saliva isolated Streptococcus Mutans, Lactobacillus and Candida Albicans via disc diffusion assay at a concentration of 200mg/mL. The minimum inhibitory concentration was carried out by the standard broth microdilution method. Cell viability of test microorganisms against different concentration of the extract and inhibition zones were calculated. Results: Disk diffusion assay showed positive zones of inhibition for all test microorganisms with S. mutans, Lactobacillus and C. albicans exhibiting zones of inhibition of 8.3 ± 0.7mm, 12.4 ± 2.4mm and 21.4 ± 2.7mm respectively. For minimum inhibitory concentration, the test microorganisms were tested at concentration of 250mg/ mL, 125mg/mL, 62.5mg/mL, 31.3mg/mL and 0mg/mL. The minimum inhibitory concentration showed that MIC of S. mutans was at 62.5mg/mL, Lactobacillus at 125mg/mL and C. albicans at 31.3mg/mL. Lastly, the cell viability results supported the MIC determined prior. Conclusion: Ethanol-based E. longifolia Jack root extract has an antimicrobial effect on the following microorganisms isolated from the saliva of high-risk caries adult patients: S. mutans, Lactobacillus and C. albicans.

10aAntimicrobial effect10aEurycoma longifolia Jack10aInhibition10aSalivary isolate1 abin Ramzi, Muhamad, Iyad1 abin Kosnin, Muhammad, Hazzim1 aFaisal, Ghasak, Ghazi1 aArzmi, Mohd, Hafiz1 aKusumawardani, Anisa1 aSabere, ASM1 aMakky, Essam, A1 aIbrahim, OE1 aFaisal, Ghasak, Ghazi1 aArzmi, Mohd, Hafiz1 aKusumawardani, Anisa1 aSabere, ASM1 aMakky, Essam, A1 aIbrahim, OE uhttps://phcogj.com/article/1426