02886nas a2200217 4500008004100000245012500041210006900166260001900235300001100254490000600265520214700271653004002418653002502458653002202483653003402505653002002539653002802559100002202587700002302609856003602632 2015 eng d00aAnti-Tyrosinase and DPPH Radical Scavenging Activities of Selected Thai Herbal Extracts Traditionally Used as Skin Toner0 aAntiTyrosinase and DPPH Radical Scavenging Activities of Selecte c27th Dec, 2014 a97-1010 v73 a

Introduction: Skin darkness may be cosmetically undesirable for some people. Plant-derived materials traditionally used for skin lightening may produce satisfactory results. Besides innate tyrosinase activity, oxidative stress also plays an important role in skin darkness by activating tyrosinase. Therefore, herbal extracts with strong anti-tyrosinase and antioxidant activities could be considered as efficacious skin lightening agents. The aims of the present study were to determine the anti-tyrosinase and antioxidant activities of Thai medicinal plant extracts indigenously used as skin toners. Methods: The activities of seven Thai medicinal plants; Zingiber cassumunar Roxb., Phyllanthus emblica Linn., Tagetes erecta Linn., Centella asiatica, Raphanus sativusvar. Longipinnatus Linn., Cassia fistula Linn. and Butea monosperma (Lam.) Taub. were investigated using the DOPA-chrome method to determine anti-tyrosinase activity and the DPPH free radical scavenging assay to determine antioxidant activity. Results: With respect to tyrosinase inhibitory activity, P. emblica, B. monosperma and R. sativusvar. Longipinnatus extracts at a concentration of 1.67 mg/mL showed strong activities with the percentage of tyrosinase inhibition at 48.38±4.77, 46.92±3.77 and 42.85±6.54 %, respectively. In addition, P. emblica and B. monosperma extracts also exhibited high antioxidant activities with the IC50values on 33.47±1.24 and 33.57±1.92 mg/mL, respectively. Conclusion: Based on tyrosinase inhibition activity and DPPH radical scavenging assays, the herbal extracts of P. emblica and B. monosperma show promise as potential skin lighteners in cosmetic formulations.

Key words: antioxidant activity, Dopachrome method, skin lightening, traditional skin toners, anti-tyrosinase inhibitory activity, DPPH radical scavenging assay.

10aanti-tyrosinase inhibitory activity10aantioxidant activity10aDopachrome method10aDPPH radical scavenging assay10askin lightening10atraditional skin toners1 aSungthong, Bunleu1 aPhadungkit, Methin uhttps://phcogj.com/article/1465