02793nas a2200241 4500008004100000245010700041210006900148260001900217300001000236490000600246520216800252653001002420653000702430653001602437653002202453100000502475700000502480700000502485700000502490700000502495700001502500856003602515 2014 eng d00aAntianaphylactic, mast cell stabilizing and antiasthmatic activity of AHR-1 (a polyherbal formulation)0 aAntianaphylactic mast cell stabilizing and antiasthmatic activit c8th April 2014 a93-990 v63 a

Objective: This work was mainly aimed to study the anti-anaphylactic, mast cell stabilizing and antiasthmatic activity of AHR-1 (a polyherbal formulation) which contain various herbal extracts. Methods: The antianaphylactic activity of AHR-1 was evaluated in rats using active anaphylaxis model. Rats were then observed for onset of symptoms of anaphylaxis reaction such as increased respiratory rate, dyspnea, cyanosis and mortality. Serum IgE, leukocyte, eosinophil countand % polymorphs were calculated. Mast cell stabilizing effect was investigated by in vitro challenge of antigensensitized rat intestinal mesenteries. Antiasthmatic effect wasstudied in guinea pigs using histamine-induced bronchospasm, in which occurrence of preconvulsive dyspnea (PCD) was noted as end point. Results: Anaphylactic shock caused by intravenous antigen challenge showed 83% mortality with a significant (P<0.001) increaserespiratory symptom score.Treatment with AHR-1(250 and 500 mg/kg) reduced the mortality and respiratory symptom score (P<0.05, P<0.001), respectively. AHR- 1 (250 and 500 mg/kg) significantly and dose dependently decreased Serum IgE (P<0.05, P<0.001), AEC (P<0.05, P<0.001), total leukocytes (P<0.05, P<0.01) and % polymorphs (P<0.01,P<0.001), respectively as compared to sensitized control group. Sensitized control rats wereproduced a significant (79%) mesenteric mast cell degranulation, but pre-treatment with AHR-1 (100 and 200μg/ml) produced in a significant (p<0.001) reduction in the number of degranulated mast cells when challenged with horse serum. AHR-1 significantly increased the time of PCD (P<0.001) as compared to control. Conclusion: From these finding, we concluded thatAHR-1 is might be effective in treatment various hypersensitivity reactions like anaphylactic shock and asthma.

Key words: Horse serum, AHR-1, IgE, Respiratory score.

10aAHR-110agE10aHorse serum10aRespiratory score1 a1 a1 a1 a1 a1 aSeth, A, K uhttps://phcogj.com/article/1484