03320nas a2200253 4500008004100000245018900041210006900230260001900299300000800318490000600326520254300332653001202875653001602887653000902903653002902912653000902941653002302950653000902973653001902982100000503001700000503006700001903011856003603030 2014 eng d00aPortulaca oleracea extract increases lecithin:cholesterol acyltransferase and paraoxonase 1 activities and enhances reverse cholesterol transport in streptozotocin-induced diabetic rat0 aPortulaca oleracea extract increases lecithincholesterol acyltra c8th April 2014 a1-90 v63 a

Background: Plant extracts are generally assumed to be more acceptable and less hazardous than synthetic compounds and could be alternative antidiabetic treatments. Portula caoleracea has been used as one of the traditional edible and medicinal plant in Algeria to treat diabetes. The aim of the present study was to determine the effects of lyophilized aqueous extract of Portulaca oleracea on high-density lipoproteins composition, paraoxonase (PON1) and lecithin:cholesterol acyltransferase (LCAT) activities in streptozotocin-induced diabetic rat. Methods: Diabetes was induced intraperitonially by a single injection of streptozotocin (STZ) (60mg/kg bw). Twelve diabetic rats, weighing 263±5g, were divided into two groups fed a casein diet supplemented or not with Portulaca oleracea extract (1g/kg bw), for 4 weeks. Results: At d28, in Portulaca oleracea treated vs untreated diabetic group, glycemia, serum total cholesterol (TC), triacylglycerols (TG) and phospholipids (PL) concentrations were decreased significantly (p<0.05). The hypolipidemic effect induced by Portulaca oleracea extract was due to the reduction of total cholesterol (TC) in LDL-HDL1 (-51%) and C-HDL3 (-21%). Portulaca oleracea treatment improved PON1 and LCAT activities by 48%. HDL3-UC (acyl group acceptor) and -PL (enzyme substrate) were diminished respectively by 47% and 82%, whereas HDL2-CE concentrations (product of LCAT reaction) were increased by 44%. Moreover, HDL-C levels were found to be positively correlated with PON1 activity (r=0.96, p<0.05). Serum, LDL-HDL1, HDL2 and HDL3 TBARS levels were respectively, 2.9-, 2.6-, 2.4- and 2.8-fold lower in Portulaca oleracea treated than untreated diabetic groups. Conclusion: These findings reflect the potential antihyperglycemic and hypolipidemic of Portulaca oleracea extract, in STZ-induced diabetic rat. Moreover, Portulaca oleracea extract restores PON1 and ameliorates the reverse cholesterol transport (RCT) by enhancing LCAT activity, therefore could prevent many diabetic complications by reducing dyslipidemia and oxidative damage.

Key words: Rats, Streptozotocin, Portulaca oleracea, Cholesterol, PON1, LCAT, apo A-I, lipoprotein peroxidation.

10aapo A-I10aCholesterol10aLCAT10alipoprotein peroxidation10aPON110aPortulaca oleracea10aRats10aStreptozotocin1 a1 a1 aSaadia, Berzou uhttps://phcogj.com/article/1495