02629nas a2200217 4500008004100000245007000041210006900111260001800180300001000198490000600208520197000214653001602184653001702200653004402217653004702261653001502308653001402323100001902337700001902356856003602375 2014 eng d00aPharmacognosy and Phytochemical Analysis of Brassica juncea Seeds0 aPharmacognosy and Phytochemical Analysis of Brassica juncea Seed c2nd July 2014 a47-540 v63 a

Introduction:Brassica juncea is an economically important plant that has been well-known in India for centuries for its medicinal and nutritive values. The broad spectrum of beneficial effects of the seeds perceived with this plant warrants further exploration of B. juncea seeds as a potential source for obtaining pharmacologically standardized phytotherapeutics, which could be potentially useful. The objective of the present study was to perform the pharmacognosy of mustards seeds inclusive of qualitative and quantitative phytochemical analysis, fingerprinting by infrared spectroscopy and high performance thin layer chromatography analysis and toxicity assessment in vitro. Methods: Different sections of seeds were taken and stained with 0.1% phloroglucinol for microscopic examination. The seeds were extracted by 80% alcohol on a rotary shaker to perform phytochemical analysis and fingerprinting. The toxicity assessment of this extract was performed on human dermal fibroblast cells. Results: Microscopic examination of seeds showed characteristic features of mustard seeds. The extraction of these seeds by 20% alcohol resulted in IC50 value of 103 ± 3 μg/mL for 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl radical scavenging assay. The fingerprinting analysis of this extract indicated probable presence of sinigrin, quercetin, vanillin, catechin, vitamin E and sulfur-containing compounds. This extract exhibited 50% toxicity (IC50) at 1.79 mg/mL. Conclusion: The result achieved will be used to assess the therapeutic efficacy of seed extracts for future pharmacological evaluations.

Key words: Antioxidant, cytotoxicity, Fourier transform infrared spectroscopy, high performance thin layer chromatography, microscopy, phenolics.

10aAntioxidant10aCytotoxicity10aFourier Transform Infrared Spectroscopy10aHigh performance Thin layer Chromatography10amicroscopy10aPhenolics1 aParikh, Harita1 aKhanna, Aparna uhttps://phcogj.com/article/1515