02673nas a2200229 4500008004100000245009000041210006900131260001800200300001000218490000600228520195400234653002902188653001402217653003402231653002602265653001702291100002302308700002802331700002302359700002502382856003602407 2014 eng d00aPharmacognostic evaluation and chrysazin quantitation of Xyris indica flowering heads0 aPharmacognostic evaluation and chrysazin quantitation of Xyris i c26th May 2014 a16-220 v63 a

Objectives: The present study aimed to establish quality specification of Xyris indica L. flowering heads. The pharmacognostic parameters were investigated. Chrysazin contents were analyzed by TLC image analysis using ImageJ software compared to TLC-densitometry. Methods: X. indica flowering heads from 15 different sources in Thailand were collected. Morphological and physicochemical parameters were characterized. Chrysazin was successively extracted and determined by TLC image analysis using ImageJ software and TLC-densitometry. Results: Macroscopic study was illustrated as whole plant drawing. The microscopic study showed fragment of corolla, seeds, pollen grain and staminode. The pharmacognostic parameters revealed that the loss on drying, total ash, acid-insoluble ash and water content should be not more than 6.90, 2.50, 0.41, and 11.12 of % dry weight respectively while water and ethanol-soluble extractive values should be not less than 6.59 and 4.03 of % dry weight respectively. TLC fingerprint revealed clearly chrysazin yellow fluorescent band at 365 nm. Chrysazin quantitation by TLC image analysis and TLC densitometry were developed and validated. Chrysazin content was 0.022 ± 0.001 % dry weight by both methods. There was no statistically significantly difference between these methods. Conclusion: This study provided pharmacognostic specification and chrysazin content of X. indica flowering heads that can be used for basic quality control and standardization of plant material. TLC image analysis using ImageJ software showed reliable and convenient for analysis of chrysazin content in this crude drug.

Key words: Xyris indica, Pharmacognostic specification, Chrysazin, Quantitative analysis, Antimicrobial activities

10aAntimicrobial activities10aChrysazin10aPharmacognostic specification10aQuantitative analysis10aXyris indica1 aKhuniad, Chuanchom1 aThitikornpong, Worathat1 aPalanuvej, Chanida1 aRuangrungsi, Nijsiri uhttps://phcogj.com/article/1524