03029nas a2200229 4500008004100000245009800041210006900139260001800208300001000226490000600236520239100242653001702633653001802650653001802668653001802686653002402704100000502728700000502733700000502738700002002743856003602763 2014 eng d00aPharmacognostical and phytochemical standardization of the roots of Potentilla mooniana Wight0 aPharmacognostical and phytochemical standardization of the roots c18th Feb,2014 a70-790 v63 a

Background:Potentilla mooniana Wight. (PM) (Family: Rosaceae) is a plant commonly grown at the higher altitudes (1500–3660 meter) of the lower Asian continent and is traditionally used to treat gastric and mouth disorders. The present study was aimed to scientifically develop a standard monograph for PM on the basis of pharmacognostical and phytochemical aspects. Methods: Pharmacognostically the roots were analyzed following the standard parameters prescribed under WHO guidelines and Indian Herbal Pharmacopoeia. Results: Morphologically, the roots are cylindrical, dark brown and astringent to bitter in taste. Histologically, the root section showed the formation of secondary growth with wood formation and central lignified xylem vessels. Physicochemical standards quantified includes foreign organic matter (1.20% w/w), loss on drying (9.66% w/w), total ash (12.65% w/w), acid insoluble ash (4.65% w/w), water soluble ash (0.5% w/w), alcohol soluble extractive (21.3% w/w), water soluble extractive (14.6% w/w), foaming index (142.85), swelling index (6.5), haemolytic index (37.77). Quantification of pesticide residue content and heavy metals such as Pb, Cd, Zn and Hg was analyzed and were found to be present within the permissible limits. Powdered drug showed the presence of lignified xylem vessels with scalariform and spiral thickenings, tracheids, starch grains and fibres. Phytochemical screening showed the presence of phenolics, tannins, flavonoids, steroids, saponins, sugars, and amino acids. Quantification of phytoconstituents were also investigated such as phenolics (84.15mg/g tannic acid equivalent, TAE), tannins (65.31mg/g TAE), flavonoids (9.53mg/g rutin equivalent, RE), flavonols (2.01mg/g RE), saponins (20.75mg/g diosgenin equivalent, DE), sapogenins (15.4mg/g DE) and carbohydrates (56.8mg/g D–fructose equivalent). TLC of the root extract was also analyzed in the present study. Conclusion: In conclusion, the diagnostic characters obtained from the roots of P. mooniana will provide beneficial information in identifying and comparing this plant from other closely related Potentilla species.

Key words:Potentilla mooniana, Pharmacognosy, phytochemical, polyphenolics, heavy metal.

10aHeavy metals10aPharmacognosy10aPhytochemical10aPolyphenolics10aPotentilla mooniana1 a1 a1 a1 aHemalatha, Siva uhttps://phcogj.com/article/1549