02108nas a2200229 4500008004100000245009500041210006900136260001800205300001200223490000600235520147000241653001501711653002001726653002101746653001601767653002301783100000501806700000501811700000501816700002101821856003601842 2014 eng d00aPharmacokinetic study of phyllanthin and hypophyllanthin after oral administration to rats0 aPharmacokinetic study of phyllanthin and hypophyllanthin after o c18th Feb,2014 a124-1300 v63 a

Objective: The present study was carried out to develop a sensitive and cost effective HPLC method for the determination of bioactive lignans (phyllanthin and hypophyllanthin) and its application in a pharmacokinetic study. Methods: Identification of lignan compounds on C–18 column was monitored at a range of 199–400nm using photodiode array detector (PDA) with methanol-water (66:34, v/v) as mobile phase at a flow rate of 1ml/min. Carbamazepine was used as internal standard. Results: From the developed method LOD and LOQ values were found to be 56.14ng/ml and 169.99ng/ml for phyllanthin, and 56.04ng/ml and 169.82ng/ml for hypophyllanthin. The validated RP–HPLC method herein was applied for pharmacokinetic studies and Cmax (ng/ml) values for administered three oral doses (2.5, 5 and 10mg/kg) of phyllanthin and hypophyllanthin were 0.28±0.06, 0.53±0.16, 0.98±0.22 and 0.68±0.76, 1.35±0.23, 2.45±0.33, respectively. Conclusion: In conclusion, developed HPLC–PDA method effectively determined the phyllanthin and hypophyllanthin in various solvent and plasma samples. This method was successfully applied in conducting their oral pharmacokinetic studies.

Key words:Phyllanthus amarus, phyllanthin, hypophyllanthin, HPLC–PDA, pharmacokinetics.

10aHPLC–PDA10ahypophyllanthin10aPharmacokinetics10aPhyllanthin10aPhyllanthus amarus1 a1 a1 a1 aDiyya, Keerthana uhttps://phcogj.com/article/1551