02322nas a2200229 4500008004100000245012700041210006900168260001400237300001200251490000700263520158000270653001601850653000901866653001701875653001401892653002401906100003501930700003701965700002902002700002502031856003602056 2021 eng d00aEvaluating the Antioxidant and Heavy Metal Content of Pleurotus ostreatus Mushrooms Cultivated using Sugar Cane Agro-Waste0 aEvaluating the Antioxidant and Heavy Metal Content of Pleurotus  cJuly 2021 a844-8520 v133 a

Background: Pleurotus ostreatus, is one of the most cultivated mushrooms with great economic and medicinal value that can be easily grown on various bio-waste substrates. However, biosafety evaluations on these mushrooms are rarely conducted. Thus, we sought to evaluate the concentration or presence of Heavy metals in P. ostreatus mushrooms cultivated on agro-bio-waste products. Furthermore, the effect of adding agro wastes on wheat bran (WB) cultivated mushrooms was evaluated. Methods: Mushrooms grown in sugar cane tops and bagasse were supplemented with varying levels of WB. Atomic absorption spectrophotometer was applied to evaluate the concentration of heavy metals in the substrates and within mushrooms. Furthermore, DPPH free radical scavenging activity was used to determine antioxidant activity of mushroom extracts. Results: The transfer factor analysis (TF) showed that mushrooms have an affinity to absorb Zn, Cd, Cu and Cr from all tested substrates during cultivation (TF>1). The addition of WB supplement into substrates resulted into significant increase in mushroom yield. However, the increased addition of WB, inversely affected the DPPH scavenging activity of the P.ostreatus methanolic extracts. Conclusion: The bioabsorption of heavy metals by P. ostreatus is depended on the metal type. Based on these findings, mushrooms grown on these agro-waste appear to be safe and potent scavenging ability against free radicals.

10aAntioxidant10aDPPH10aHeavy metals10aMushrooms10aPleurotus ostreatus1 aMkhize, Senzosenkosi, Surprise1 aSimelane, Mthokozisi, Blessing C1 aGasa, Nothando, Lovedale1 aPooe, Ofentse, Jacob uhttps://phcogj.com/article/1603