02178nas a2200253 4500008004100000245010100041210006900142260001400211300001200225490000700237520141000244653000601654653003201660653002501692653002001717653002601737653001801763100000501781700002601786700002101812700003401833700002101867856003601888 2021 eng d00aPhytochemicals and Antioxidant Activities Evaluation of Origanum vulgare (L.) Stem Bark Extracts0 aPhytochemicals and Antioxidant Activities Evaluation of Origanum cJuly 2021 a965-9700 v133 a

The present study aimed to evaluate phytochemical and antioxidant activity (in vitro and in vivo) of Origanum vulgare (L.) ethanolic extract. The phytochemical test was assessed using the Clule method in ethanol, ethyl acetate, and hexane. In vitro evaluation of antioxidant activity was determined by radical scavenging assay using DPPH (2,2-diphenyl-1-picrylhydrazyl) as an artificial free radical activity. In vivo test was conducted to evaluate the effect of malondialdehyde (MDA) level in blood plasma during maximum physical activity treatment. In vivo test was done using 25 male Sprague Dawley rats in pre and post-test control group design. The phytochemical test of O. vulgare ethanol extract was showed some compounds, such as a flavonoid, alkaloid, triterpenoid/steroid, essential oil, and tannin, then in ethyl acetate and hexane. In vitro assay showed that O. vulgare extract has strong antioxidant activity with an IC50 value of 133.47 μg/mL. While in the in vivo test, the most effective dosage is 20 mg/200 gr B.W., represented by a significant decrease of MDA level (0.509 nmol/mL) before and after treatment. So, the ethanolic extract of clove has potency as an herbal antioxidant because of the low level of IC50 and can decrease the MDA level.

10a210a2-diphenyl-1-picrylhydrazyl10aantioxidant activity10aMalondialdehyde10aOriganum vulgare (L.)10aPhytochemical1 a1 aWidyastuti, Putu, Ayu1 aYazid, Fatmawaty1 aAmbarwati, Neneng, Siti Silfi1 aAhmad, Islamudin uhttps://phcogj.com/article/1619