02732nas a2200241 4500008004100000245010700041210006900148260001400217300001400231490000700245520195200252653002302204653001802227653002902245100003202274700002702306700002602333700003302359700002502392700002102417700001602438856003602454 2021 eng d00aCytotoxic Activity of Eurycoma Longifolia Jack Root Extract Against Nasopharyngeal Carcinoma Cell Line0 aCytotoxic Activity of Eurycoma Longifolia Jack Root Extract Agai cJuly 2021 a1014-10180 v133 a

Background: Eurycoma longifolia (E. longifolia) or Tongkat Ali is a tree that grows in southeast Asia, the roots of which contain bioactive components that exhibit cytotoxic properties against various cancer cell lines. However, no study has been conducted to relate the cytotoxic properties against nasopharyngeal carcinoma (NPC), a type of cancer that shows poor prognosis for metastatic disease. The purpose of this study was to determine whether the E. longifolia root extract exerts cytotoxic activity against nasopharyngeal carcinoma (ORL-115) cell lines. Materials and Method: E. longifolia root extracts were obtained through Soxhlet extraction method and by using two different solvents; ethanol and dicholoromethane. MTS assay was used to evaluate the cytotoxic effect of the root extracts against ORL-115 cell line for three different incubation time which were 24-hour, 48-hour and 72-hour. Results: Ethanol extract was significantly more potent compared to DCM extract. Ethanol extract exhibited lower IC50 value compared to DCM extract. The IC50 of ethanol extract were 232.1 μg/ml, 66.86 μg/ml and 42.6 μg/ml. Meanwhile the IC50 of DCM extract were 678.87 μg/ml, 136.71 μg/ml, 73.72 μg/ml for 24-hour, 48-hour and 72-hour incubation period respectively. The cytotoxic activity of both extracts increased as the incubation time prolonged. The cytotoxic activity of ethanol extract at each incubation time was significantly different from DCM extract except at 72 hours. Conclusion: E. longifolia root extracts exerted cytotoxic activity against the nasopharyngeal carcinoma (ORL-115) cell line. Ethanol extract exhibited lower IC50 value compared to DCM extract. The cytotoxic activity of both extracts were dose dependent and time dependent.

10aCytotoxic activity10aE. longifolia10aNasopharyngeal carcinoma1 aKajahmohideen, Nur, Haseena1 aRazi, Siti, NurSyafiqa1 aFaisal, Ghasak, Ghazi1 aAshour, Abdelkader, Elbadawy1 aKusumawardani, Anisa1 aMakky, Essam, A.1 aIbrahim, OE uhttps://phcogj.com/article/1626