02744nas a2200229 4500008004100000245011200041210006900153260001900222300001400241490000700255520201100262653002502273653001702298653002902315653001902344100002502363700001802388700002402406700002502430700002302455856003602478 2021 eng d00aPhytochemical and Biological Characterization of Aqueous and Ethanolic Extracts of Parthenium hysterophorus0 aPhytochemical and Biological Characterization of Aqueous and Eth cSeptember 2021 a1122-11330 v133 a

Introduction: Parthenium hysterophorus is a plant used in traditional medicine to treat health issues and which could be a source of phytochemicals with possible antioxidant activity without causing cytotoxic effects. Hence, this work was designed to evaluate its phytochemical profile, cytotoxicity, and antioxidant activity. Methods: The aqueous (AE) and ethanolic (EE) extracts of P. hysterophorus flowers were obtained by decoction and ultrasound, respectively. Their phytochemical composition was determined by colorimetric tests and RP-HPLC-MS analysis. Their cytotoxic activity was tested by a hemolysis assay. The antioxidant activity was evaluated with the Trolox equivalent antioxidant capacity (TEAC), 2,2-diphenyl-1- picrylhydrazyl (DPPH), and hydroxyl radical (-OH) scavenging assays. In addition, the effect of the extracts on the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) from human erythrocytes, was evaluated. Results: The phytochemical screening of the AE and EE by colorimetric test showed the presence of flavonoids, steroids, triterpenes, saponins, coumarins, sesquiterpene lactones, tannins, and carbohydrates. In addition, the RP-HPLC-MS analysis identified some phenolic compounds such as flavonols, methoxyflavonols, flavones, methoxyflavones, and hydroxycinnamic acids. The hemolysis assay showed non-cytotoxic activity by AE, but EE exhibited a hemolytic effect. Furthermore, the AE and EE showed significant antioxidant activity to inhibit radicals in the TEAC, DPPH and -OH scavenging assays. Moreover, the SOD activity only showed a significant increase by AE. However, the two crude extracts increased the CAT activity, at the highest concentrations. Conclusion: P. hysterophorus has phytochemicals with antioxidant activity to inhibit radicals and increase the activity of antioxidant enzymes in vitro.

10aantioxidant activity10aCytotoxicity10aParthenium hysterophorus10aPhytochemicals1 aJiménez, MA, Alfaro1 aCruz, Zugasti1 aBelmares, SY, Silva1 aValdés, JA, Ascacio1 aRivera, CA, Sierra uhttps://phcogj.com/article/1641