02646nas a2200241 4500008004100000245016500041210006900206260001800275300001400293490000700307520179600314653002802110653002602138653003102164653003902195653002102234100002302255700003202278700002002310700002202330700001602352856003602368 2021 eng d00aThe Effect of Mahkota Dewa (Phaleria macrocarpa) Leaf Extract on the Mucin 1 Expression in Mice Colonic Epithelial Cells Induced by Dextran Sodium Sulfate (DSS)0 aEffect of Mahkota Dewa Phaleria macrocarpa Leaf Extract on the M cNovember 2021 a1509-15150 v133 a

Background: Inflammatory bowel disease is a chronic inflammation caused by the malignant inflammation response and if not treated, could lead to colorectal cancer. One of the researched treatment is mahkota dewa (Phaleria macrocarpa) leaf extract that has flavonoid compound known to reduce inflammation. This study was aimed to prove that mahkota dewa leaf extract could reduce inflammation of mice colon induced with dextran sodium sulfate (DSS) and observe MUC1 expression from colon epithelial crypt of Lieberkuhn. Methods: This was a laboratory experiment using biological material (paraffin block) taken from 28 mice and divided into 5 groups: normal, aspirin, low and high dose mahkota dewa, and negative control. They were processed into immunohistochemistry and stained microscopic slides. Afterwards, they were observed with 400x magnification and 5 field-of-view of mice colon crypt of lieberkuhn. Then MUC1 expression was counted using ImageJ to obtain mean immunohistochemistry score and analyzed with SPSS. Results: There were significant reduction of MUC1 expressions from normal, aspirin, and high dose mahkota dewa groups compared to the negative control group. The result shown MUC1 expression from high dose mahkota dewa (M=149.90,SD=3.81) and aspirin (M=158.92,SD=5.28) were closer to normal group (M=148.02,SD=5.28). There were no significant results between negative (M=175.39,SD=14.30) and low dose mahkota dewa group (M=149.90,SD=5.02). Conclusion: There was a reduction of MUC1 expression in DSS-induced mice colonic epithelial cells for high dose mahkota dewa group. This shown that high dosage mahkota dewa leaf extract could reduce inflammation like aspirin.

10aAnti-inflammatory agent10aColon epithelial cell10aInflammatory bowel Disease10aMahkota Dewa (Phaleria macrocarpa)10aMUC 1 expression1 aKusmardi, Kusmardi1 aSitumorang, Natasha, Yemima1 aZuraidah, Endah1 aEstuningtyas, Ari1 aTedjo, Aryo uhttps://phcogj.com/article/1691