01938nas a2200205 4500008004100000245010400041210006900145260001800214300001400232490000700246520133700253653002001590653002501610653001101635653001801646100000501664700000501669700002201674856003601696 2021 eng d00aAntioxidant Activity and Phytochemical Identification of Annona Squamosa Leaves Methanolic Extracts0 aAntioxidant Activity and Phytochemical Identification of Annona  cDecember 2021 a1746-17500 v133 a

The antioxidant activity of Annona squamosa (A. squamosa) leaf was carried out using methanol extract and fractionated extract namely n-hexane, ethyl acetate and residue. The antioxidant activity of methanol extract and fractionated was performed using the 1-diphenyl-2-Pycrilhydrazil (DPPH) method. The antioxidant activity test of methanol extract and fractionated n-hexane, ethyl acetate and residue yielded IC50 of 6.87, 169.99, 31.55 and 44.75 ppm. The ethyl acetate fraction extract with IC50 31.55 ppm was performed by column chromatography using silica gel G60 as the stationary phase and n-hexane: ethyl acetate as the mobile phase. The results of column chromatography obtained 181 fractions and were combined based on the stain pattern into 4 subfractions. Antioxidant test of each subfraction showed that the ASE 3 subfraction had the strongest antioxidant activity. Furthermore, the subfraction was analyzed using Gas Chromatography Mass Spectrometry (GC-MS). According, GC-MS data analysis showed that the third subfraction contained 19 phytochemical compounds, where 3 compounds having the highest concentration, namely 4,4'-((p-Phenylene)diisopropylidene) diphenol, dodecanoic acid, methyl ester and phthalic acid, isobutyl 2-methylpent-3-yl ester.

10aAnnona squamosa10aantioxidant activity10aGC-MS.10aPlant extract1 a1 a1 aGinting, Binawati uhttps://phcogj.com/article/1720