02628nas a2200229 4500008004100000245012300041210006900164260001800233300001400251490000700265520189600272653002002168653002302188653001602211653001102227100002502238700002102263700002702284700002502311700002602336856003602362 2021 eng d00aMolecular Study of Acalypha indica to Leptin, Alpha Glucosidase, and its Antihyperglycemic Effect on Alpha Glucosidase0 aMolecular Study of Acalypha indica to Leptin Alpha Glucosidase a cDecember 2021 a1639-16470 v133 a

Introduction: The purpose of this study is to find potential inhibitors of leptin as a proinflammatory adipokine and alpha glucosidase as an enzyme that mediate hyperglycaemia; to alter the chronic complications of obesity from herbal Acalypha indica (Ai). This study was conducted using in silico molecular docking to evaluate the Ai compounds interaction with leptin and alpha glucosidase. The in vitro assay to alpha glucosidase was done to explore antihyperglycemic effect of Ai, as hyperglycaemia is the key process of chronic complication of obesity. Material and Methods: Protein target were leptin and alpha glucosidase; compounds from Ai plant were repundusinic, mauritanin, hesperetin, acaindinin, and glucogalin in pdb format. Molecular docking using autodock vinna. In vitro assay of Ai antihyperglycemic activity was done to alpha glucosidase and was define as IC50 level. Result: The results from the docking analysis demonstrated that compounds from Ai roots contain antihyperglycemic-antiobesity activity which acted by inhibiting leptin and alpha glucosidase receptors. Repundusininc and mauritanin compounds contain hydrogen bond with the greatest leptin enhancer activity on Ser9, Thr35, Glu8, Ser9, Thr25, Gln111, Lys211, Leu7 for repundisinic and Glu8, Thr25, Gly112 and Leu7 for mauritanin. Hesperetin, acaindinin and glucogallin were the most identical compounds with similar affinity binding value to alpha glucosidase. Ai roots was already proven as anti-hyperglycemic-antiobesity which was further confirmed by in vitro assay to alpha glucosidase (IC50 19,429 μg/ml.). Conclusion: The results demonstrated that Ai have anti hyperglycaemic-antiobesity effects and was found to be potentially as antihyperglycemic by in vitro assay to alpha glucosidase.

10aAcalypha indica10aAlpha glucosidase.10aAntiobesity10aLeptin1 aHakim, Rani, Wardani1 aFadilah, Fadilah1 aTarigan, Tri, Juli Edi1 aJusman, Sri, Widia A1 aPurwaningsih, Erni, H uhttps://phcogj.com/article/1727