02176nas a2200157 4500008004100000245011300041210006900154260001800223300001200241490000700253520165500260100002701915700002001942700002001962856003601982 2022 eng d00aValidation of Rosmarinic Acid Quantification using High- Performance Liquid Chromatography in Various Plants0 aValidation of Rosmarinic Acid Quantification using High Performa cFebruary 2022 a165-1710 v143 a

Introduction: Rosmarinic acid has been utilized in traditional medicine as antioxidant, antiinflammation, anticancer and antibacterial. In order to control the herbal quality, validation of rosmarinic acid determination using high-performance liquid chromatography was developed. The objective of this report was to validate an HPLC technique for assessing rosmarinic acid levels. and application that method to determine rosmarinic acid in Rosmarinus officinalis, Symphytum officinale, Mentha piperita, Orthosiphon stamineus and Salvia officinale. Methods: The chromatographic separation was carried out on a reversed-phase C18 column with a mobile phase of 0,1% formic acid and acetonitrile and an isocratic elution at a flow rate of 0,5 mL/min. The wavelength for detection was set to 330 nm. The method has been validated for precision, accuracy, linearity, limit of detection, and limit of quantitation. Result: The concentration response of the detector was linear, with a coefficient of determination of 0.9933. The HPLC technique had an accuracy of 101,00 ± 6,43%. The precision was 6,36% when expressed as a coefficient of variation (CV). The highest level of rosmarinic acid was 214,86 ± 0,60 μg/mL in Rosmarinus officinalis extract. Conclusion: The HPLC method was valid to analyse rosmarinic acid level. The method can be applied in routine determination of rosmarinic acid of phytopharmaceutical products.

Key words: Rosmarinic acid, HPLC, Laminaceae Borraginaceae.

1 aSyarifah, Andiri, Niza1 aSuryadi, Herman1 aMun’im, Abdul uhttps://phcogj.com/article/1750