01936nas a2200181 4500008004100000245011000041210006900151260001800220300001200238490000700250520135000257100002801607700001801635700002601653700001801679700002101697856003601718 2022 eng d00aAntimicrobial Compound from Endophytic Pseudomonas azotoformans UICC B-91 of Neesia altissima (Malvaceae)0 aAntimicrobial Compound from Endophytic Pseudomonas azotoformans  cFebruary 2022 a172-1810 v143 a

Centrifugated extract of endophytic Pseudomonas azotoformans strain UICC B-91 has been observed for its antimicrobial activity against Escherichia coli ATCC 8739, Bacillus cereus ATCC 10876, Staphylococcus aureus ATCC 6583, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 25241, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659 and Candida albicans ATCC 10231, with minimum concentration in the range 250 μg/mL. But, the novel compound of the bioactive substances which relevant for antimicrobial activity have not been described. Further fractionation and identification of the extract by thin layer chromatography (TLC) and liquid chromatography mass spectrophotometry (LCMS-MS) detected several compound as futoamide (C18H23NO3), gentialutine (C9H11NO), gentiabetine (C9H11NO2), 1-[(2E,4E)-2,4-decadienoyl]pyrrolidine (C14H23NO), lycopodine (C16H25NO) and dihydrolycopodine (C16H27NO). The majority compounds containing CHON elements with molecular weight approximately 100-450 Da.

Keywords: Alkaloid, Antimicrobial compound, Extraction, P. azotoformans.

1 aPratiwi, Rina, Hidayati1 aOktarina, Eva1 aMangunwardoyo, Wibowo1 aHidayat, Iman1 aSaepudin, Endang uhttps://phcogj.com/article/1751