02207nas a2200217 4500008004100000245015400041210006900195260001500264300001200279490000700291520151300298653001401811653001601825653002001841653001101861100002101872700001401893700002701907700001901934856003601953 2022 eng d00aPreliminary Identification and Quantification of Four Secondary Metabolites, Total Tannin and Total Flavonoid Contents in Guava Fruit Ethanol Extract0 aPreliminary Identification and Quantification of Four Secondary  cApril 2022 a350-3570 v143 a

Introduction: The study on guava fruit ethanol extract from Dukuhwaluh Village, Purwokerto, Central Java, Indonesia showed increased megakaryocytes and platelet numbers in thrombocytopenic mice model. The study of acute oral toxicity of the extract did not show toxic effects in the kidney and liver at doses of 2000 and 5000 mg/kg b.w. The aim of the study was to determine the profile and quantity of four metabolite compounds and total tannin and flavonoid in the extract. Materials and Method: gallic acid, ellagic acid, rutin and kaemferol in the extract were identified and quantified by using high-performance liquid chromatography (HPLC) method with column LiChroCART 250-4,6 RP 18E, isocratic mobile phases with the composition of mixture:0.1% acetic acid, acetonitrile and methanol (40:50:10) respectively, and at temperature 300C. The total tannin and flavonoid were determined using the by spectrophotometry method, Fe(III) chloride and 1.10-phenanthroline at wavelength 510 nm for tannin and aluminium chloride and rutin at wavelength 422 nm for flavonoid, respectively. Results: Percentage of gallic acid, ellagic acid, rutin and kaempferol were 0.77%, 1.37 %, 0.41 % and 0.35 %, respectively. Total tannin and flavonoid contents were 1.20% (TAE) and, 1.18% (RE) respectively. Conclusion: The guava fruit ethanol extract contained gallic acid, ellagic acid, rutin, kaempferol, tannin and flavonoid.

10aFlavonoid10aGuava fruit10aPsidium guajava10aTannin1 aDhianawaty, Diah1 aAtik, Nur1 aDwiwina, Resti, Gradia1 aMuda, Iskandar uhttps://phcogj.com/article/1774