02470nas a2200205 4500008004100000245014400041210006900185260001400254300001200268490000700280520177400287653003202061653002302093653002202116653001902138100003302157700002202190700001602212856003602228 2022 eng d00aAzadirachta indica Hexane Extract: Potent Antibacterial Activity Against Propionibacterium acne and Identification of its Chemicals Content0 aAzadirachta indica Hexane Extract Potent Antibacterial Activity  cJune 2022 a489-4960 v143 a

Background: Acne is a skin surface disease that appears when the excessive fat deposits clogged the skin pores, causes the growth of acne-causing bacteria and stimulates inflammation. Propionibacterium acnes is one of common acne-causing bacteria which usually manage by synthetic chemical-based drug. However, the presence of its long- used side effects pointed the urgent need of new anti P. acne drug discovery. Azadirachta indica is a medicinal plant which empirically used as antibacterial. A. indica leaves has been reported to exhibit activity against P. acne but limited to ethanol extract. Thus, the evaluation of other extract- and identification of active compound(s) against P. acne is needed to be explore. Methods: First, the microscopic morphology of A. indica leaves were observed using Scanning Electron Microscope. The leaves were then extracted sequentially by hexane, ethyl acetate, and methanol solvent using the ultrasonic assisted extraction method, followed by its in vitro anti- P. acne activity evaluation. The most active extract was further evaluated for its chemical(s) content by LC-MS. Results: Scanning Electron Microscope identified the presence of oxalate in the leaves of A. indica. Evaluation of the anti-P. acne activity showed that the hexane extract had highest anti-P. acne compared to others. Further chemical identification showed that hexane extract contains three steroids, one saturated acids and one phenolic compounds. Conclusions: A. indica hexane extract leaf is prospective to be developed as an acne antibacterial.

10aAnti-Propionibacterium acne10aAzadirachta indica10aChemical content.10aHexane extract1 aSilvyana, Annysa, Ellycornia1 aRahmasari, Ratika1 aElya, Berna uhttps://phcogj.com/article/1794