02578nas a2200253   4500008004100000245014300041210007000184260001400254300001200268490000700280520179600287653001702083653002302100653001002123653000902133653001402142653001202156100002502168700001802193700002202211700002102233700003402254856003602288       2022                        eng d00aEfficacy of Probiotic on Duodenal TNF-α Expression and the Histological Findings in the Liver and Lung in Animal Model Canine Coronavirus0 aEfficacy of Probiotic on Duodenal TNFα Expression and the Histol  cJune 2022  a591-5970 v143 a<p class="rtejustify">Currently, Canine coronavirus (CCoV) is an enteric pathogen of the Alphacoronavirus-1 species that causes mild to severe diarrhea in puppies. The pathogenesis of this infection will cause severe lymphopenia and lead to death in puppies. This study aimed to determine the administration of probiotics on TNF-α expression, histological findings of the liver and lung in mice infected with CCoV. A total of 28 mice were randomly assigned into seven treatment groups, i.e. (C-) placebo; (C+) active CCoV vaccine induction; (T1) CCov + Isopronosin; (T2) CCoV + Lactobacillus acidophilus probiotic; (T3) CCoV + Lactobacillus Acidophylus and Bifidobacterium probiotics; (T4) CCoV + colustrum fermentation probiotic; (T5) CCoV + ginger, turmeric and ginger probiotics. Thereafter, the expression of TNF-α in the duodenum was stained using immunohistochemistry, liver and lung were stained using hematoxylin eosin. The data were analyzed using the ANOVA test followed by the Tukey test with a significance level (p&lt;0.05). TNF-α expression on T4 and T5 decreased significantly (p&lt;0.05) compared to C+, T1, T2 and T3. Histologic findings of the liver in the C- and T4 groups showed normal features in the central vein. On the other hand, glycogen accumulation was found in hepatocyte cells, hemorrhage with sinusoid dilation, lymphocyte infiltration in centro lobular area in group C+. Lung histology showed normal features of sinusoids and alveolar septa in groups C- and T4. Meanwhile, intra-alveolar hemorrhage was found with neutrophil cell infiltration and fibrin plasma accumulation in group C+. In conclusion, colostrum fermentation probiotics can reduce TNF-α expression in the duodenum and improve the liver and lung physiology in mice infected with CCoV.</p>
10aBiodiversity10aCanine coronavirus10aLiver10aLung10aProbiotic10aTNF-α.1 aHamid, Iwan, Sahrial1 aEkowati, Juni1 aSolfaine, Rondius1 aChhetri, Shekhar1 aPurnama, Muhammad, Thohawi El  uhttps://phcogj.com/article/1806