02644nas a2200301 4500008004100000245011700041210006900158260001400227300001200241490000700253520160400260653002301864653002101887653001901908653001301927100003801940700003201978700003202010700003402042700003502076700003202111700003802143700003302181700002702214700003702241700002802278856003602306 2022 eng d00aPharmacobotany, Phytochemical Analysis and Anti-inflammatory effect of the Ethanolic Extract of Luffa operculata0 aPharmacobotany Phytochemical Analysis and Antiinflammatory effec cJune 2022 a622-6280 v143 a

Background: Luffa operculata is a vegetable species well known in the traditional peruvian medicine for its many medicinal properties and cosmetic applications. Objective: The aim objective was to determine the pharmacognostic characteristics of L. operculata as well as observing the pharmacological effect of the ethanol extract of L. operculata fruit on chronic inflammation in rats. Materials and Method: Phytochemical analysis was carried out by using specific chemical reagents for each constituent chemical, the pharmacobotanical study was done with a histological tinction (fruit, steam and leaves), which were stained with Safranin 1% and Toluidine blue 1%; the chronical inflammation was assessed by air bag method in Holztman male rats. Doses of 100, 250 and 500 mg/Kg were tested in order to determine the anti-inflammatory effect, which was demonstrated with histopathological evaluation and lymphocytes reduction. Results: The main findings indicate that the ethanolic extract presented saponins, alkaloids, carbohydrates, terpenes and steroids. The efficiency of lymphocyte reduction per field in the histopathological study of the granuloma was 58.4% with the middle dose of 250 mg/kg (p < 0.0001), which gives a dose-independent anti-inflammatory effect in rats. Conclusion: Luffa operculata presented anti-inflammatory effect at 250 mg/Kg by oral administration in a chronical experimental model of inflammation in rats.

10aAnti-inflammatory.10aLuffa operculata10aPharmacobotany10aSaponins1 aArroyo-Sandoval, Jorge, Alejandro1 aMarin-Bravo, Manuel, Jesús1 aArroyo-Acevedo, Jorge, Luis1 aJustil-Guerrero, Hugo, Jesús1 aChávez-Asmat, Roberto, Jesús1 aPari-Olarte, Josefa, Bertha1 aChávez-Espinoza, Javier, Hernán1 aTorres-Lévano, Jaime, David1 aLoyola-Gonzales, Eddie1 aAlmeida-Galindo, José, Santiago1 aHerrera-Calderon, Oscar uhttps://phcogj.com/article/1810