02516nas a2200253 4500008004100000245008700041210006900128260001400197300001200211490000700223520181500230653001602045653001302061653001202074653000902086653001102095653001502106653001002121100001902131700003502150700002102185700002002206856003602226 2022 eng d00aMangosteen (Garcinia mangostana L.): Evaluation of In Vitro Antioxidant Activities0 aMangosteen Garcinia mangostana L Evaluation of In Vitro Antioxid cJune 2022 a633-6400 v143 a

Introduction: Mangosteen (Garcinia mangostana L.), is an evergreen of the Guttiferae family that carries antioxidant activity. Objectives: to examine the antioxidant activity of the leaves, branches and rinds of the mangosteen using DPPH and CUPRAC methods, total phenolic content (TPC) and total flavonoid content (TFC), analyze the correlation between TPC, TFC and antioxidant activity, the correlation between two methods, and found the levels of flavonoid compounds. Methods: Extraction was performed by reflux method using solvents with graded polarity, namely n-hexane, ethyl acetate and ethanol. Determination of antioxidant activity with DPPH and CUPRAC, TPC and TFC were performed by UV-visible spectrophotometer. The correlation between TPC, TFC and antioxidant activity of DPPH and CUPRAC as well as the correlation between two methods were conducted by Pearson’s method. The level of flavonoid compounds was performed by HPLC. Results: Mangosteen leaves, branches and rinds extracts had antioxidant activity of DPPH in the range of 39.920 – 489.708 mg AAE/g and antioxidant activity of CUPRAC in the range of 116.360 – 570.400 mg AAE/g. The highest TPC was given by the ethanol leaves extract (49.525 ± 4.263 g GAE/100 g) and the highest TFC was given by the n-hexane rinds extract (13.859 ± 1.451 g QE/100 g). The ethanol rinds extract contained rutin 0.0327% and kaempferol 0.0049%. Conclusions: TPC and TFC correlated positive and significant with the value of antioxidant activity, except for the n-hexane leaves extract using the DPPH method. The DPPH and CUPRAC methods gave linear results in determining the antioxidant activity of mangosteen extracts.

10aAntioxidant10aBranches10aCUPRAC.10aDPPH10aLeaves10aMangosteen10aRinds1 aRizaldy, Defri1 aRamadhita, Nisrina, Khairunnis1 aNadhifa, Trishna1 aFidrianny, Irda uhttps://phcogj.com/article/1814