02205nas a2200277 4500008004100000024000700041245017100048210006900219260001700288300001200305490000700317520131800324653001701642653001601659653002601675653003601701100001901737700002301756700001901779700001801798700000501816700002501821700001901846700002601865856003601891 2022 eng d a0600aAntioxidant Activity of DPPH, CUPRAC, and FRAP Methods, as well as Activity of Alpha-Glucosidase Inhibiting Enzymes from Tinospora crispa (L.) Stem Ultrasonic Extract0 aAntioxidant Activity of DPPH CUPRAC and FRAP Methods as well as  cOctober 2022 a511-5200 v143 a

Aims: The goal of this work was to get the stem extract of Tinospora crispa (L.) using ultrasoundassisted extraction (UAE) with duration and amplitude changes to achieve optimal extraction conditions. The antioxidant potential of the extract as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), CUPRAC (Cupric Ion Reducing Antioxidant Capacity), FRAP (Ferric Reducing Ability of Plasma), and its antidiabetic potential. Results: The third test of antioxidant methods showed that the measurement of antioxidant activity with, DPPH, CUPRAC, and FRAP gave significantly different results, but CUPRAC and FRAP gave the same response to the ethanol extract of Tinospora crispa stems, it can be concluded that the extract of Tinospora crispa stems in all treatments had strong antioxidant activity using either the CUPRAC or methods FRAP. In addition, it is very active as an antidiabetic by inhibiting alpha glucosidase. The optimum extract selected was extract B (extraction time was 35 minutes and amplitude 65%) with IC50 values for antioxidant activity in the CUPRAC method of 72.53 ± 0.18 mg/L and the FRAP method of 152.29 ± 2.16 mg/L, while the inhibition of alpha glucosidase was 0.30 ± 0.006 mg/L.

10aAntidiabetic10aAntioxidant10aTinospora crispa (L.)10aUltrasound-assisted extraction.1 aIrawan, Candra1 aPutri, Imalia, Dwi1 aSukiman, Maman1 aUtami, Andita1 a1 aPutri, Ratna, Komala1 aLisandi, Anisa1 aPratama, Andrean, Nur uhttps://phcogj.com/article/1863