02418nas a2200289 4500008004100000024000700041245013500048210007000183260001800253300001200271490000700283520147800290653001701768653001601785653001801801653002301819653002601842100002601868700002601894700003101920700003001951700003001981700002602011700003102037700002402068856003602092 2022 eng d a1200aChemical Profiling and In Vitro α-amylase Antidiabetic Assessment of Carissa Macrocarpa Flower Extract Cultivated in Saudi Arabia0 aChemical Profiling and In Vitro αamylase Antidiabetic Assessment cDecember 2022 a759-7650 v143 a

Carissa macrocarpa is commonly known as Natal plum. Its fruits are palatable and used in manufacture of jams while other parts of the plant are used in traditional medicine to treat various diseases. The main objective of current study was to screen the presence of and isolate the various phytochemicals applying standard procedures and to evaluate the in vitro antidiabetic activity using α-amylase inhibitory assay. The total methanol extract of flower (TMEF) of Carissa macrocarpa was subjected to several chromatographic procedures. Results demonstrated that TMEF of Carissa macrocarpa is characterized by the content of different constituents such as flavonoids, steroids, saponins, tannins and carbohydrates at different levels. Chromatographic isolation led to the isolation of kaempferol-3-O-robinobioside and caffeic acid, which were confirmed via using 1H, 13C, DEPT, COSY, HMQC and HMBC NMR spectroscopic analyses. TMEF exhibited α-amylase inhibitory activity with IC50 value of 65.4 μg/ml when compared to that of the acarbose (standard) (IC50 = 39.6 μg/ml). In conclusion, current investigation endorses the traditional use of Carissa macrocarpa as antidiabetic herb. Hence, the studied TMEF of Carissa macrocarpa may have the potential being nutraceuticals products for pharmaceutical applications as antidiabetic herbal remedy.

10aAntidiabetic10aApocynaceae10aCaffeic acid.10aCarissa macrocarpa10aα-amylase inhibitory1 aAlghafli, Duaa, Adnan1 aAlbahrani, Zeneb, Ali1 aAlnasser, Fatemah, Hussain1 aAlnajdi, Aldanah, Ibrahim1 aAlanazi, Gharam, Mohammed1 aBurshed, Hussein, Ali1 aAlshawush, Marwan, Mohamed1 aKhalil, Hany, Ezzat uhttps://phcogj.com/article/1891