02570nas a2200289 4500008004100000024000600041245018100047210006900228260001700297300001200314490000700326520158800333653000901921653002601930653001701956653002001973653001901993100002702012700003402039700002602073700003502099700002902134700002602163700003102189700002402220856003602244 2022 eng d a900aPhytochemical Approach Including Total Phenolic and Flavonoid Contents and Evaluation of in vitro ABTS Antioxidant Capacity and Lipoxygenase Inhibition of Anisosciadium lanatum0 aPhytochemical Approach Including Total Phenolic and Flavonoid Co cJanuary 2023 a928-9320 v143 a

Anisosciadium lanatum Boiss is commonly known in Bedouins as besbas. Traditional, it is palatable plant and used in medicine of livestock to treat skin conditions . The main objective of current approach was to screen the presence of different kind of metabolites applying standard procedures followed by assessment of total phenolic (TPC) and flavonoids (TFC) contents. In addition, the in vitro ABTS antioxidant and lipoxygenase activities were evaluated. Different organs (leaves, stems and flowers) of Anisosciadium lanatum were extracted using 70% methanol to yield total methanol extracts of leaves (TML), stems (TMS) and flowers (TMF). Results demonstrated that TML, TMS and TMF are characterized by the content of different constituents such as flavonoids, phenolics/tannins, steroids, saponins, and carbohydrates at different levels. Ethyl acetate (EA) and butanol (BT) fractions of TML and TMS demonstrated the highest percentage of TPC and TFC. The results demonstrated the competence of EA and BT as free radical scavenger fractions compared to other fractions and its opportunity to contain bioactive antioxidant metabolites . TML, TMS and TMF exhibited Lipoxygenase inhibitory activity with IC50 values of 4.88, 5.40 and 6.05 μg/mL, respectively when compared to that of the positive control baicalein (IC50: 0.27 μg /mL). In conclusion, present investigation highlighted the potential of Anisosciadium lanatum to be promising candidate with activity against wide range of inflammatory-related diseases.

10aABTS10aAnisosciadium lanatum10aLipoxygenase10atotal flavonoid10atotal phenolic1 aMatar, Mohammed, Wasel1 aAlqahtani, Shahad, Mohammed N1 aAlghafli, Duaa, Adnan1 aAltaweel, Abdullah, Abdulhamid1 aAlasoom, Abdullah, Jalal1 aBurshed, Hussein, Ali1 aAlshawush, Marwan, Mohamed1 aKhalil, Hany, Ezzat uhttps://phcogj.com/article/1894