02215nas a2200265 4500008004100000024000700041245012100048210006900169260001700238300001200255490000700267520136000274653002101634653001701655653002301672653001101695100003401706700002801740700002901768700002601797700003101823700003501854700002401889856003601913 2022 eng d a1000aCichorioside a biocoumarin modulates lipid and glucose storage on 3T3-L1 cell lines: In vitro and in silico approach0 aCichorioside a biocoumarin modulates lipid and glucose storage o cJanuary 2023 a933-9370 v143 a

Natural coumarins are reputed to demonstrate several biological effects to improve adverse health conditions including; obesity. This study was conducted to explore the potential in vitro lipid and glucose modulatory activity of cichorioside on 3T3-L1 cell lines . Methods: The 3T3-L1 cell lines were cultured and cell viability was assessed. Glucose content in medium of cultured cells was measured. Differentiation of 3T3-L1 cells from pre-adipocytes to adipocytes was evaluated upon addition of cichorioside. Expression of mRNA of the peroxisome proliferator-activated receptor gamma (PPARγ) was estimated. Results: Survival of around 90% of cultured cells was observed at 10μM cichorioside. Cichorioside inhibited glucose uptake from the medium by the 3T3-L1 cell lines. Cichorioside considerably inhibited pre-adipocyte differentiation and the lipid content in intercellular storage. Cichorioside demonstrated an upregulation of the mRNA expression of PPARγ. Moreover, the docking studies supported the results via the deep interaction of cichorioside with amino acids residue of PPAR-γ. Taken together, these findings are the first report on in vitro evaluation of cichorioside to modulate the lipid storage and glucose uptake of cultured 3T3-L1 cell lines

10a3T3-L1cell lines10aCichorioside10aOil red O staining10aPPARγ1 aAlqahtani, Shahad, Mohammed N1 aAlsuliman, Dalya, Ramzi1 aAlasoom, Abdullah, Jalal1 aBurshed, Hussein, Ali1 aAlshawush, Marwan, Mohamed1 aAltaweel, Abdullah, Abdulhamid1 aKhalil, Hany, Ezzat uhttps://phcogj.com/article/1895