02579nas a2200229 4500008004100000024000700041245022600048210006900274260001700343300001400360490000700374520175700381653002202138653002402160653001002184653001702194653001702211653003702228100002202265700002602287856003602313 2022 eng d a2600aBio-Evaluation, In-Vitro and In-Vivo Anti-Inflammatory Activity, Therapeutic Efficacy, and Genotoxicity of the Potentials of the Green Seaweed Valoniopsis Pachynema using Zebra Fish Larvae (Danio Rerio) as an Animal Model0 aBioEvaluation InVitro and InVivo AntiInflammatory Activity Thera cJanuary 2023 a1037-10530 v143 a

Advancement in the medical sectors to treat regular diseases are increasing day-by-day. Yet, there is a considerable growth in the demand for the natural/herbal products as well due to their low level of side effects, cost efficiency and their multiple inhibition properties. Based on this, the present research works with an objective to examine the bioactive components, in vitro anti-inflammatory and in vivo antiinflammatory behaviour of the green marine macro algae Valoniopsis pachynema using zebra fish (Danio rerio) larvae as a skin inflammation model. In this study, the secondary metabolites are extracted using methanol solvent from the marine green seaweed, V. pachynema using the Gas Chromatography-Mass Spectrometry (GC-MS) analysis and these are further evaluated for their anti-inflammatory effects. Further screening process is accomplished for the in vitro anti-inflammatory activity by the albumin-denaturation inhibition. Results from concentration-dependent analysis is documented. The efficacy, therapeutic efficacy, and genotoxicity of the compound Valp at various concentrations are determined by recapitulating the pathophysiology of Skin inflammation in Zebrafish larvae. In evaluating the efficiency of the study, Valp at 1 pg, 10 pg, 100 pg are observed and progressed for the evaluation of therapeutic efficacy and genotoxicity. In the assessment of genotoxicity, the gene expression of mgmt gene is observed to be in control level at Valp 100 pg treated group confirming no genotoxicity. According to the results obtained, the green seaweed V. pachynema can be potentially explored as an effectual anti-inflammatory agent for its bio-functionalities

10aAnti-inflammatory10aBioactive compounds10aGC-MS10aMarine algae10aV. pachynema10aZebra fish larvae drug toxicity.1 aJ, Bhuvaneshwari.1 aP, Thirumalai, Vasan. uhttps://phcogj.com/article/1947