02285nas a2200253 4500008004100000024000700041245009000048210006900138260001500207300001000222490000700232520155400239653000901793653001401802653001901816653001601835653001501851100002901866700002401895700002601919700002901945700002101974856003601995 2023 eng d a0600aThe effect of Sinensetin and Imperatorin on A-549 lung cancer cell viability in vitro0 aeffect of Sinensetin and Imperatorin on A549 lung cancer cell vi cMarch 2023 a38-460 v153 a

Introduction: Lung cancer remains the leading cause of cancer death worldwide, so research is ongoing to discover new therapeutics, such as plant-derived bioactive compounds. For example, Sinensetin, a plant-derived polymethoxylated flavonoid, and Imperatorin, a natural furanocoumarin, have anti-cancer properties. This study assessed the effects of sinensetin and imperatorin separately and in combination on A-549 lung cancer cell viability. Method: The A-549 lung cancer cell line was treated with sinensetin (60 μM), imperatorin (30 M), or a combination of both compounds (Sin:Imp 30:30 μM; 50:50 μM and 60:30 μM) for 48 hours. Cell viability was then assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and apoptosis was determined using fluorescein isothiocyanate (FITC) Annexin-V/Propidium iodide staining. Results: The combination treatment of Sin:Imp 50:50 and Sin:Imp 60:30 μM reduced cell viability more than the individual treatment of sinensetin and imperatorin, with the lowest cell viability observed for the combination treatment of Sin:Imp 50:50 μM. Likewise, the combination treatment of Sin:Imp 50:50 μM induced the most apoptosis compared to individual treatment. Conclusion: Sinensetin and imperatorin can decrease A-549 lung cancer cell viability and are potent apoptotic inducers, especially when they are used in combination, therefore they are potential lung cancer therapeutics.

10aA54910aApoptosis10aCell viability10aImperatorin10aSinensetin1 aIndriyanti, Raden, Anita1 aAriyanto, Eko, Fuji1 aUsman, Hermin, Aminah1 aEffendy, Ristaniah, Rose1 aDhianawaty, Diah uhttps://phcogj.com/article/1958