02721nas a2200277 4500008004100000024000700041245015100048210006900199260001400268300001200282490000700294520184100301653002302142653000902165653002502174653003302199653002202232100002102254700003002275700001902305700002002324700000502344700003002349700002802379856003602407 2023 eng d a0200aOptimization of Xanthine Oxidase Activity, Phytochemical Screening, Toxicity Assay, and Antigout Activity of Spatholobus littoralis Hassk. Extract0 aOptimization of Xanthine Oxidase Activity Phytochemical Screenin cJune 2023 a258-2690 v153 a

Introduction: A medicinal plant from Central Kalimantan, Spatholobus littoralis Hassk., with a local name bajakah tampala, has long been used to treat gout or uric acid disease. This study aimed to develop the optimum conditions of xanthine oxidase (XO) activity, phytochemical screening, and to obtain the antigout activity of S. littoralis Hassk. Methods: Before performing the phytochemical screening, we performed a simplicia maceration with water and 70% ethanol solvents. The development of the optimum conditions for XO was carried out using the Response Surface Methodology (RSM) combined Box-Behnken Design (BBD). Toxicity assay (LC50) on both extracts was assessed by BSLT (Brine Shrimp Lethality Test). The two extracts were then evaluated for antigout activity. Results: The yield of 70% ethanol extract (11.24%) was higher than the aqueous extract (7.29%). The phytochemical screening on the simplicia and the two extracts positively contained steroids, flavonoids, saponins, and tannins. The optimum conditions of XO activity were temperature of 20 ℃, pH of 8.0, and xanthine concentration of 1.40 mM. The toxicity of the 70% ethanol extract (LC50 662.25 mg/L) was stronger than the aqueous extract (LC50 774.68 mg/L). Moreover, the 70% ethanol extract (IC50 224.14±8.62 mg/L) showed higher XO inhibition than the water extract (IC50 348.83±4.85 mg/L). Lineweaver-Burk plot analysis showed that 70% ethanol and water extracts inhibited XO competitively with the inhibition constant (KI) of 272.68 mg/L and 378.44 mg/L, respectively. Conclusion: This preliminary study can be a reference that S. littoralis Hassk. is potentially an antigout.

10aBox-Behnken Design10aGout10aLineweaver-Burk plot10aSpatholobus littoralis Hassk10aXanthine Oxidase.1 aRahminiwati, Min1 aSianipar, Rut, Novalia Ra1 aSutriah, Komar1 aIswantini, Dyah1 a1 aAchmadi, Suminar, Setiati1 aSulistyawan, Ibnu, Hari uhttps://phcogj.com/article/2028