02285nas a2200217 4500008004100000024000700041245008600048210006900134260001400203300001200217490000700229520163900236653002501875653002501900653001201925653002001937100002301957700002901980700002202009856003602031 2023 eng d a1200aMetabolite Profiling of Compounds from Sargassum polycystum using UPLC-QToF-MS/MS0 aMetabolite Profiling of Compounds from Sargassum polycystum usin cJune 2023 a321-3330 v153 a

Background: There are many types of seaweed that have high economic value. Brown seaweed (Sargassum polycystum) can be used as a raw material in the industry and as a medicinal plant. Maintaining the quality of a compound requires an analytical method that can identify the diversity of metabolome profiles. Objective: This investigation seeks to discover the metabolite profile of S. polycystum from Sumenep, Madura Island, Indonesia, utilizing the UPLC-QToF MS/MS equipment. Materials and Methods: The extract was further fractioned using n-hexane, ethyl acetate, and water. The metabolite profiling of extract and fractions used the UPLC-QToF-MS/MS instrument. It was produced with SPE and then introduced into the MS Xevo G2-S QToF detector of the ACQUITY UPLC® H-Class System. The findings of the UPLC-QToF-MS/MS analysis were processed with the MassLynx 4.1 software to obtain chromatogram data and m/z spectra of each observed peak, which were then validated using the ChemSpider and MassBank databases. Results: Based on the results of metabolite profiling using UPLC-QToF-MS/MS, the 96 % ethanol extract of S. polycystum indicated a total of 61 compounds, the n-hexane fraction indicated a total of 55 compounds, the ethyl acetate fraction indicated a total of 67 compounds, and the water fraction indicated a total of 49 compounds. Conclusion: There are 232 compounds in the extract and a fraction of S. polycystum consisting of 168 known compounds and 64 unknown compounds.

10aMetabolite profiling10aSargassum polycystum10aSeaweed10aUPLC-QToF-MS/MS1 aRiwanti, Pramudita1 aPrasetyanti, Intan, Kris1 aMa’arif, Burhan uhttps://phcogj.com/article/2038