03166nas a2200385   4500008004100000024000700041245019600048210006900244260001700313300001200330490000700342520174100349653005002090653002302140653001402163653006102177653006202238653005502300653001302355653001402368653003302382653003502415653001302450653001202463653001802475653003302493653006402526653001102590653004902601653000802650100002802658700002902686700002902715856003602744       2023                        eng d  a1000aAnti-Neoplastic and Cytotoxicity Potency Measuring of Five Medicago sativa L. (Alfalfa) Leaf Extracts Towards Melanoma (UACC62), Breast (MCF7), Prostate (PC3), and Colon (HCT116) Cancer Cells0 aAntiNeoplastic and Cytotoxicity Potency Measuring of Five Medica  cOctober 2023  a768-7760 v153 a<p class="rtejustify">The National Cancer Registry of the National Department of Health of South Africa confirms that in females, the highest incidence of cancers diagnosed in the year 2019 was breast (10 172 cases), cervical (6 945 cases) and colorectal (1 952 cases) cancers, while in males the most diagnosed cancers were prostate, lung, and colorectal neoplasms. The risk factors that promote cancer development, and metastasis or spreading includes excessive alcohol and narcotic usage, carcinogenic infections (oncoviruses) and genetical predispositions. The global incidence of cancer is much likely to increase because of the increased exposure to risk factors (processed fast foods, industrial toxins in air and water) that promote oxidative stress, low grade chronic inflammation, diabetes mellitus, hyperglycemia, and insulin resistance. Because the ancient healer or doctor relied on plants and other natural resources for therapeutic compounds, we wanted to measure the anti-cancer actions induced by our Alfac-facah leaf extracts towards four major cancer cells. The growth inhibitory and cytotoxicity activity that was induced by our extracts was measured using the reliable Sulforhodamine B Assay as per the collaborative research program between the CSIR’s Biosciences Pharmacology Group and the NCI. Our Alfac-facah leaf test material was extracted using five solvents: Ethanol, Methanol, Diethyl-ether, Acetone, and Water. Medicinal Plants persist to play a key role in medicine, whereby they not only help with treating or preventing diseases, but they also contribute to the general wellbeing of the patient. For this reason, natural resources remain a pivotal ingredient of novel drug development compounds</p>
10aAICR (American Institute for Cancer Research)10aCancer (malignancy10aCarcinoma10aCSIR (The Council for Scientific and Industrial Research10aECACC (European Collection of Authenticated Cell Cultures10aInternational Agency for Research on Cancer (IARC)10aLeukemia10alymphoma)10aMedicago sativa L. (Alfalfa)10aNCI (National Cancer Institute10aneoplasm10asarcoma10aSouth Africa)10aSulforhodamine B Assay (SRB)10aTHC (delta-9 tetrahydrocannabinol). Rick Simpson oil (RSO).10aTumour10aU.S Department of Health and Human Services)10aUK)1 aSimon, Makhele, Thapelo1 aMoses, Makhoahle, Pakiso1 aSamson, Mashele, Sitheni  uhttps://phcogj.com/article/2116