02535nas a2200241 4500008004100000024000700041245013400048210006900182260001700251300001200268490000700280520174900287653002202036653002202058653002502080653002302105100002802128700002502156700002302181700002502204700002802229856003602257 2023 eng d a2700aThe Investigation of The Network Pharmacology and Mechanism of Action of Centella Asiatica Extract on The Atopic Dermatitis Model0 aInvestigation of The Network Pharmacology and Mechanism of Actio cOctober 2023 a881-8900 v153 a

Background: Atopic dermatitis (AD) is a chronic relapsing inflammatory skin condition which has a negative impact on children health. The well-known medicinal plant Centella asiatica extract (CE) is used in herbal skin care products to produce various pharmacological effects in dermatology. However, the molecular target of CE in suppressing inflammatory is largely unknown. Objective: the aim of this study was to examine anti-inflammatory properties and network pharmacology of CE in lipopolysaccharide (LPS)- induced AD in vitro model. Method: RAW264.7 cells were pre-treated with CE and then were stimulated with LPS and then were investigated cell viability, NO production, and the levels of pro-inflammatory mediators. In addition, the Search Tool for Retrieval of Interacting Genes (STRING), SwissTargetPrediction and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to construct the defined mechanism of action and network pharmacology. Results: CE showed the potent inhibitory effects on LPS-induced NO. In addition, CE significantly suppressed the expression of iNOS and COX-2, as well as the production of IL-2, IL-6, IL-10, and TNF- α. Furthermore, the network pharmacological analysis revealed the potential role of CE in biological processes such as regulating JAK/STATs pathway and inhibiting proinflammatory cytokines both of which were linked to AD pathogenesis. Conclusion: Our findings confirm our hypothesis that CE could be developed as a therapeutic therapy for atopic dermatitis due to its pharmacological action and signaling mechanism in the modulation of allergic skin inflammation.

10aAtopic dermatitis10aCentella asiatica10aNetwork pharmacology10aSkin inflammation.1 aTungsukruthai, Sucharat1 aPochairach, Runtikan1 aKrajarng, Aungkana1 aJumpa-ngern, Piracha1 aTungsukruthai, Parunkul uhttps://phcogj.com/article/2135