02562nas a2200253   4500008004100000024000700041245010500048210006900153260001700222300001200239490000700251520182000258653000902078653001602087653001902103653001902122100001802141700003302159700002702192700001802219700001902237700001602256856003602272       2023                        eng d  a2800aHydrocephalus Mice Model: Choroid Plexus Aquaporin-1 Dynamics Following Cerebrospinal Fluid Drainage0 aHydrocephalus Mice Model Choroid Plexus Aquaporin1 Dynamics Foll  cOctober 2023  a891-8960 v153 a<p class="rtejustify"><strong>Background:</strong> Aquaporins (AQPs) are a family of membrane proteins that act as channels for water, facilitating its movement across the plasma membrane of cells. Aquaporin1 (AQP1), located in the choroid plexus, is thought to be involved in the process of cerebrospinal fluid (CSF) production. Objective: The objective of this study is to examine the impact of hydrocephalus and cerebrospinal fluid (CSF) drainage on the expression of AQP1 in a mice model of hydrocephalus.<strong> Material and Methods</strong>: Laboratory experimental study with six groups. Five test groups, one control group, and a rat model of hydrocephalus caused by kaolin were used in the experiment. <strong>Results:</strong> Hydrocephalus in mice model induced by kaolin, and CSF drainage was performed on the 7<sup>th</sup> and 14<sup>th</sup> days group. Immunohistochemical analysis was conducted to examine the presence of AQP1 in the<em> choroid plexus</em> using microscopes. The findings revealed a noticeable decrease in AQP1 expression levels in the <em>choroid plexus,</em> which exhibited a semi-quantitative decline in correlation with the duration of hydrocephalus (p = 0.01). This decrease was observed when comparing the normal group with the hydrocephalus groups on the 7<sup>th</sup>, 14<sup>th</sup>, and 21st days following induction. However, after cerebrospinal fluid (CSF) drainage, there was a significant increase in AQP1 expression (p &lt; 0.05). <strong>Conclusions: </strong>This study shows the significant role of AQP1 in CSF production by comparing of AQP1 expression in the<em> choroid plexus </em>of hydrocephalus mice model, with and without CSF drainage. AQP1 expression experiences downregulation in hydrocephalus mice model and upregulation after CSF drainage.</p>
10aAQP110aAquaporin 110aChoroid plexus10aHydrocephalus.1 aBaktir, Yusuf1 aParenrengi, Muhammad, Arifin1 aSuryaningtyas, Wihasto1 aFauziah, Dyah1 aSudiana, Ketut1 aUtomo, Budi  uhttps://phcogj.com/article/2136