01943nas a2200217 4500008004100000024000700041245009100048210006900139260001700208300001200225490000700237520128500244653001301529653001401542653003301556653001501589653003101604100002501635700002901660856003601689 2023 eng d a3200aGeneration of Multinucleated Giant Cells due to Leishmania (V.) braziliensis Infection0 aGeneration of Multinucleated Giant Cells due to Leishmania V bra cOctober 2023 a905-9090 v153 a

Background: Multinucleated giant cells were first described by Langhans(MGCs), these MGCs have a role in innate immunity that includes extracellular matrix remodeling associated with granuloma formation; another function of them is to participate in the removal of cellular debris in apoptosis during certain infections. Objective: Generate multinucleated giant cells due to Leishmania (V.) braziliensis infection. Methods: For infection macrophage/parasite ratio of 1 to 10 using the RAW 264.7-line (5 x105/mL) and Leishmania (V.) braziliensis metacyclic promastigotes (50 x105/mL) was a strain maintained in culture Schneider liquid medium, supplemented with 20% fetal bovine serum and 10 000 U/10 mg/mL penicillin streptomycin at pH 7 and 25 °C in the laboratory. Incubation was continued for 4 days, and microscopic observation at 1000X was performed at 24 hours and 96 hours, respectively. Results: In our study an MGC conversion was observed at 96 hours (50% ± 28.2). Conclusions: In vitro MGC model could be used to study the physiopathology of MGC generation by Leishmania (V.) braziliensis infection.

10aImmunity10aInfection10aLeishmania (V.) braziliensis10aMacrophage10aMultinucleated Giant Cells1 aRojas-Jaimes, Jesús1 aPelaez, Luis, Castañeda uhttps://phcogj.com/article/2139