02816nas a2200205 4500008004100000245017400041210006900215260001800284300001400302490000700316520212400323653001002447653002302457653000802480653001202488100003002500700001802530700002602548856003602574 2023 eng d00aConformity of Differential White Blood Cell (WBC) Examination Results on the Sysmex DI-60 And Sysmex XN-1000 at Prof. Dr. IGNG Ngoerah General Hospital, Denpasar, Bali0 aConformity of Differential White Blood Cell WBC Examination Resu cDecember 2023 a1059-10630 v153 a

Background: Sysmex DI-60 and Sysmex XN-1000 are automatic analyzers that can be used to check WBC differential counts. Ensuring the suitability of the WBC differential count results from the two tools can help in daily practice. Objective: Knowing the suitability of the WBC differential count examination results on the Sysmex DI-60 and Sysmex XN-1000. Method: Observational analytical research with a cross-sectional design. The data analyzed were differential WBC count data from samples of babies who underwent Complete Blood Count examinations on the Sysmex XN-1000 device and IT Ratio on the Sysmex DI-60 device without intervention (pre-classification results) at the Clinical Pathology Laboratory, Prof. Head overMarch 2023 – April 2023. Results: 81 samples were analyzed. The neutrophil value on the Sysmex DI-60 and Sysmex vs 34.15% ± 14.83%, median monocytes 4.00% (0.00-30.00)% vs 13.05% (1.30-34.40) %, median eosinophils 3.50% (0.00-31.00)% vs 3.75% (0.00-29.90)%, and median basophils 0.75% (0.00-16.00)% vs 0.30% (0, 00-2.90)%. The eosinophil variable did not show a significant difference between the results of the Sysmex DI-60 and Sysmex XN-1000 examinations (p=0.081). The results of the correlation analysis showed that there was a significant, strong, positive correlation between Sysmex DI60 and Sysmex. Strong positive significance in the lymphocyte variable (r=0.818). However, no significant correlation basophil variable (r=0.044). Bland-Altman analysis shows that the most appropriate differential count examination is the eosinophil variable. Conclusion: Automatic WBC analyzers apply not only one examination method to detect WBC. The use of the Sysmex DI-60 and Sysmex XN-1000 provides automation for checking the WBC differential count with different working principles. In this study, there was only agreement between the differential count results for the eosinophil variable, so when operating these tools, intervention from a Clinical Pathologist was still required.

10aDI-6010aDifferential Count10aWBC10aXN-10001 aCahyadi, Dewa, Komang Agu1 aWande, Nyoman1 aMahartini, Ni, Nyoman uhttps://phcogj.com/article/2162