02915nas a2200277 4500008004100000245017100041210006900212260001800281300001000299490000700309520196900316653003302285653001402318653003302332653002202365653002002387653002402407100003302431700002802464700002702492700001802519700001902537700001602556700002902572856003602601 2024 eng d00aEffects of Cerebrospinal Fluid Drainage on Pro-Inflammatory and Anti-Inflammatory Cytokines Expression in the Subventricular Zone of Kaolin-Induced Hydrocephalic Rats0 aEffects of Cerebrospinal Fluid Drainage on ProInflammatory and A cFebruary 2024 a20-270 v163 a

Background: To determine the neuroprotective effect of CSF drainage by analyzing its impact on the expression and the ratio of pro- and anti-inflammatory cytokines in the subventricular zone in kaolininduced hydrocephalic rats. Method: Sprague-Dawley rats of 23 weeks of age (n=36) were used in this study. The rats were randomly divided into normal control, hydrocephalus, and CSF drainage-treated groups. Hydrocephalus was obtained by injecting 0,05 cc of 20% kaolin suspension into the cisterna magna. The CSF drainage-treated group had ventricular tapping seven days after kaolin induction. The rats were sacrificed 7, 14, or 21 days after kaolin induction. The brain was removed and prepared for immunohistochemistry analysis to detect IL-1β, IL-6, TNF-α, and IL-10 cytokines expression. Results: Immunohistochemistry analysis revealed that the expression of pro-inflammatory cytokines was significantly increased in hydrocephalus groups than in the control group. In contrast, the expression of anti-inflammatory cytokine was significantly decreased. CSF drainage had a neuroprotective effect by reducing pro-inflammatory cytokine expression and increasing anti-inflammatory cytokine expression. In the hydrocephalus group, the ratios of IL-1β/IL-10, IL-6/IL-10, and TNF-α/IL-10 increased toward a pro-inflammatory status. After CSF drainage, the ratios of IL-1β/IL-10, IL-6/IL-10, and TNF-α/IL-10 shifted toward an anti-inflammatory status. Conclusion: CSF drainage protects the brain from excessive neuroinflammatory processes in kaolin-induced hydrocephalic rats. Additional investigation is warranted to ascertain the use of inflammatory cytokines expression as a valuable biomarker for hydrocephalus. Furthermore, research on anti-inflammatory drug administration in clinical settings is required.

10acerebrospinal fluid drainage10aCytokines10aKaolin-induced hydrocephalus10aNeuroinflammation10aNeuroprotective10asubventricular zone1 aParenrengi, Muhammad, Arifin1 aDariansyah, Ahmad, Data1 aSuryaningtyas, Wihasto1 aFauziah, Dyah1 aSudiana, Ketut1 aUtomo, Budi1 aGunawan, Prastiya, Indra uhttps://phcogj.com/article/2204