02643nas a2200253 4500008004100000245011900041210006900160260001800229300001200247490000700259520183000266653001402096653001502110653002402125653003302149653001602182100002102198700003002219700002502249700002802274700003002302700002102332856003602353 2024 eng d00aBioguided Assay of Polyphenols Isolated from Medicinal Mayan Species and its Activity Against Leishmania mexicana.0 aBioguided Assay of Polyphenols Isolated from Medicinal Mayan Spe cFebruary 2024 a174-1800 v163 a

Objective: This study underlines the in vitro leishmanicidal activity of the methanol extracts (MeOH), fractions of n-hexane (n-Hex), chloroform (TCM) and ethyl acetate (EtOAc), and compounds isolated from plant species used in the Mayan traditional medicine. Materials and Methods: Extracts of medicinal species collected in the Mayan Peninsula such as Hylocerus undatus, Bauhinia divaricate, Euphorbia hirta, Ruellia nudiflora and Cedrela odorata, were tasted in a bio guided assays against amastigotes of Leishmania mexicana. Different chromatographic techniques were applied in order to isolated the most active compounds. Additionally, spectroscopic experiments 1H-NMR, 13C-NMR, LC-MS and FT-IR were stablished to determine the chemical structure of the chemical compounds. Results: Euphorbia hirta and Cedrella odorata, showed good bioactivity with 14.81 ± 2.63 g/mL and IC50 = 18.39 ± 0.88 μg/mL respectively, meanwhile Bauhinia divaricata not show activity and Ruellia nudiflora showed poor activity with IC50 = 92.18 ± 3.64 μg/mL, followed by Hylocerus undatus with IC50 = 122.5 ± 20.99 μg/mL, when tasted against amastigotes of Leishmania mexicana. Spectroscopic data confirmed the presence of quercetin, myricetin, kempherol and scopoletin, with IC50 = 2.92 ± 0.42 μM, 12.30 ± 0.57 μM, 20.22 ± 4.66 μM and 4.05 ± 0.68 μM respectively. Conclusion: The bioguided assays guided us, to the purification and isolation of four different metabolites, mainly flavonoids and structurally related compounds, some of them show good activity, however, their low bioavailability indicates the need for detailed structural relation activity studies, together with the development of formulations and delivery systems.

10aCoumarins10aFlavonoids10aLeishmania mexicana10aNMR structural determination10aPolyphenols1 aLarqué, Horacio1 aMontes, Abelardo, Chávez1 aZamora-Chimal, Jaime1 aLooh-Hernández, Moises1 aLuevano, Joel, H. Elizond1 adel Olmo, Esther uhttps://phcogj.com/article/2225