02338nas a2200229   4500008004100000245010700041210006900148260001700217300001400234490000700248520163100255653001401886653001701900653001101917653002701928653002001955100002101975700002901996700002202025700002502047856003602072       2024                        eng d00aIn Vitro Antiviral Activity of Dried Red Jujube Fruit (Ziziphus jujuba) Ethanol Extract against DENV-20 aIn Vitro Antiviral Activity of Dried Red Jujube Fruit Ziziphus j  cOctober 2024  a1047-10500 v163 a<p class="rtejustify">Dengue infection has become one of the most important mosquito-borne diseases worldwide and is caused by the dengue virus (DENV). Recently, neither patent drug, phytopharmaceutical medicine, nor standardized herbal medicine has been officially available against DENV. Dried red jujube fruit <em>(Ziziphus jujuba)</em> ethanol extract has been proven to have an antiviral effect, anti-inflammatory efficacy, and antioxidant properties, which have potential activity against DENV infection. This research was conducted to analyze the antiviral activity of dried red jujube fruit ethanol extract against DENV-2 in vitro. The halfmaximal cytotoxic concentration (CC<sub>50</sub>) and half-maximal inhibitory concentration (IC<sub>50</sub>) were examined on Vero cells by a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, whereas the halfmaximal effective concentration (EC<sub>50</sub>) was determined using luminescence assay. The selectivity index (SI) value was determined from the ratio of CC<sub>50</sub> and EC50. Dried red jujube fruit ethanol extracts inhibited DENV-2 in 24.59%, 22.39%, 17.98%, 14.5%, 6.42%, and 1.28% at 80 μg/mL, 40 μg/mL, 20 μg/mL, 10 μg/ mL, 5 μg/mL, and 2.5 μg/mL, respectively. The extract exhibited antiviral activity against DENV-2, showing a CC<sub>50</sub> of 67.73 μg/mL, an IC<sub>50</sub> value of 166.18 μg/mL, and an EC<sub>50</sub> of 64.87 μg/mL, with an SI of 1.04. The LD<sub>50</sub> value was 707.95 mg/kg. Dried red jujube fruit ethanol extract could be a potential candidate for developing an antiviral against DENV-2.</p>
10aAntiviral10aDengue virus10aDENV-210adried red jujube fruit10aZiziphus jujuba1 aSuwasanti, Niluh1 aTedyanto, Cecilia, Putri1 aSutandhio, Silvia1 aSucipto, Teguh, Hari  uhttps://phcogj.com/article/2381