02564nas a2200181 4500008004100000245012600041210006900167260001700236300001400253490000700267520187400274653009802148100002502246700002802271700002002299700002702319856003602346 2024 eng d00aEffect of Tender Coconut Water Powder Enriched with Vitamin E in Preventing Oxidative Stress of Diabetes Male Wistar Rats0 aEffect of Tender Coconut Water Powder Enriched with Vitamin E in cOctober 2024 a1062-10680 v163 a

Background: Diabetes mellitus (DM) is associated with increased oxidative stress and higher mortality rates. Analysis is needed to identify compounds in natural foods that can prevent oxidative stress. According to previous studies, tender coconut waterwith added vitamin E is more effective in preventing oxidative stress caused by DM compared to plain tender coconut water. This development is necessary to provide added value and practicality. Objective: To evaluate the potential of tender coconut water powder inriched with vitamin E on male rats diabetes by measuring the oxidative stress (SOD, GPx, MDA, IL-1, IL-6, TNF-α, and CRP levels). Methods: An experimental method was used with a posttest control group design. A total of 36 male Wistar rats used were randomly divided into 6 groups namely normal; DM; P1 (type 2 DM + Spray Drying tender coconut water powder); P2 (type 2 DM + Spray Drying tender coconut water powder + vitamin E); P3 (type 2 DM + Freeze Drying tender coconut water powder); and P4 (type 2 DM + Freeze Drying tender coconut water powder + vitamin E). Type 2 DM induction was carried out using Streptozotocin (STZ) 65 mg/kgBW and Nicotinamide 230 mg/kgBW. Tender coconut water powder was administered daily for 4 weeks beginning on the 3rd day after inducing DM. The data obtained were analyzed using the One Way Analysis of Variance test (ANOVA). Result: The results showed that the average levels of SOD and GPx in P4 were higher compared to P2, while MDA, IL-1, IL-6, TNF α, and CRP in P4 were lower compared to P2. Conclusion: Tender coconut water powder enriched with vitamin E effectively prevented oxidative stress, as indicated by increased SOD and GPx, along with decreased MDA, IL-1, IL-6, TNF α, and CRP levels.

10aTender coconut water powder; oxidative stress; Diabetes mellitus; spray drying; freeze drying1 aRatnawati, Ratnawati1 aZulaikhah, Siti, Thomas1 aHussaana, Atina1 aPratama, Arrizki, Azka uhttps://phcogj.com/article/2384