02824nas a2200277 4500008004100000245020800041210006900249260001800318300001400336490000700350520189600357653001002253653000802263653002002271653002502291653002302316100002302339700002002362700002402382700001702406700002002423700002102443700002602464700002002490856003602510 2024 eng d00aThe Effect Ethanol Extract of Phyllanthus niruri l on Malondialdehyde (MDA) Expression and Extracellular Signal- Regulated Protein Kinase-1 (ERK-1) on Vaginal Epithelial Cell Thickness in Menopausal Mice0 aEffect Ethanol Extract of Phyllanthus niruri l on Malondialdehyd cDecember 2024 a1305-13100 v163 a

Introduction: Menopause is the condition of the ovaries stops produce estrogen so that resulting in vaginal bleeding atrophy that is become dry cause pain moment relate sexual so that lower quality life. The ROS pathway with MAPK regulates proliferation, differentiation, motility, and survival cell life. Research purposes is know influence giving extract Phyllanthus niruri l against Malondialdehyde (MDA) expression and Extracellular Signal-Regulated Protein Kinase-1 (ERK-1) expression against thickness cell vaginal epithelium of menopausal model mice. Material from Phillantus niruri l processed become extract. Treatment animal try mice (mus muscullus) first acclimatized during one next week done ovariectomy of both ovaries, after two weeks checked vaginal examination to be sure phase diestrus (menopause). Stage treatment given extract for 21 days with dose different 14 mg, 28 mg and 56 mg/20gBW/ day. Methods: True Experimental research method with Post Test only with control group design. Data analysis used one way ANOVA. Results: The research group that produced the highest average expression of Malondialdehyde (MDA) was the control group. The highest expression of Extracellular Signal-Regulated Protein Kinase-1 (ERK-1) was in the P3 treatment group (dose 56 mg/20gBW/day). The results of statistical analysis showed that there was a significant effect of Phyllanthus niruri l extract on decreasing MDA expression with a sig value of 0.000 < p = 21 0.001 and increasing ERK-1 with a sig value of 0.000 < 0.001, but there was no effect on increasing the thickness of the vaginal wall epithelial cells in menopausal model mice. with a sig value of 0.220 > 0.05. Conclusion: The three doses of phillantus niruri decreased MDA and increased ERK-1. The Folin-Ciocalteau.

10aERK-110aMDA10aMenopausal Mice10aPhyllanthus niruri l10aVaginal epithelium1 aFadliyah, Lailatul1 aHendarto, Hendy1 aSudaryanti, Lestari1 aSusilo, Imam1 aMa’ruf, Anwar1 aSulpat, Emuliana1 aWijayanti, Endah, Sri1 aSeptriana, Maya uhttps://phcogj.com/article/2424