02521nas a2200253 4500008004100000245014800041210006900189260001800258300001400276490000700290520169600297653001001993653002102003653001502024653001602039653001102055100002602066700003702092700003002129700002502159700003102184700001602215856003602231 2024 eng d00aEffect of Cacao Bean Extract as a Paracetamol Adjuvant on Pain Scale and Tumor Necrosis Factor-Alpha in Neuropathic Pain: An Animal Model Study0 aEffect of Cacao Bean Extract as a Paracetamol Adjuvant on Pain S cDecember 2024 a1336-13410 v163 a

Introduction: One treatment for neuropathic pain is paracetamol. Meanwhile, cacao bean extract is a traditional remedy developed for pain management. Objective: Analyzing effect of combining cacao bean extract and paracetamol on pain scale and tumor necrosis factor-alpha (TNF-α) in neuropathic pain. Methods: Subjects were randomized post-test only control group design from 28 mice (Mus musculus) to 4 groups: G0 (control), G1 (paracetamol only), G2 (cacao + paracetamol), and G3 (cacao + ½ doses paracetamol). The subject assessed pain scale using von Frey test and TNF-α. The statistical analysis includes paired t-tests, Wilcoxon, one-way ANOVA, Kruskal Wallis, and Pearson correlation tests with p <0.05. Results: The combination of cacao bean extract and paracetamol resulted in a pain scale of 2.57 ± 1.10 gf, with significant differences observed among the four groups (p <0.001). Significant differences in pain scale scores were found in four groups (p <0.001), including G0 (p = 0.006), G1 (p <0.001), G2 (p <0.001), and G3 (p <0.001). After treatment, the average TNF-α levels was 86.96 ± 23.73 ng/mL, with significant differences observed among the four groups (p <0.001). There was a strong correlation between the pain scale and TNF-α levels (p <0.001). Conclusion: In an animal model of neuropathic pain, using cacao bean extract as a paracetamol adjuvant significantly reduces pain scale (as measured by the von Frey test) and TNF-α levels.

10aCacao10aNeuropathic pain10apain scale10aParacetamol10aTNF-α1 aAmmar, Faiz, Muhammad1 aWaloejo, Christrijogo, Sumartono1 aPutri, Herdiani, Sulistyo1 aSantoso, Kohar, Hari1 aAirlangga, Prananda, Surya1 aUtomo, Budi uhttps://phcogj.com/article/2429