03183nas a2200229 4500008004100000245012300041210006900164260001600233300001200249490000700261520247400268653001802742653001602760653001902776653001102795653001802806100002302824700002202847700002102869700002702890856003602917 2025 eng d00aComparative Phytochemical, Antioxidant, and Antibacterial Study of Different Solvent Extracts of Cissus hastata Leaves0 aComparative Phytochemical Antioxidant and Antibacterial Study of cAugust 2025 a511-5190 v173 a

Introduction: Plants have long been utilized in traditional medicine for treating a variety of diseases. Plant-derived extracts are a rich source of phytochemicals with documented antibacterial and antioxidant properties. Objective: This study aimed to investigate and compare the phytochemical profiles, antioxidant capacities, and antibacterial activities of various crude extracts from the leaves of Cissus hastata. Methods: Leaf extracts of C. hastata were prepared using hexane (Hex), ethyl acetate (EtOAc), 95% ethanol (EtOH), and 50% ethanol (AqE). These extracts were analyzed for preliminary phytochemical constituents, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (DPPH, ABTS, and FRAP assays), and antibacterial activity against five bacterial strains: Staphylococcus aureus (TISTR 517), methicillin-resistant Staphylococcus aureus (MRSA 142), Bacillus cereus (ATCC 11778), Escherichia coli (ESBL 182), and Salmonella typhimurium (TISTR 292). Results: Phytochemical screening of different solvent extracts of C. hastata leaves revealed the presence of diverse bioactive compounds. The EtOAc and AqE extracts exhibited the highest TPC (65.31 ± 1.85 and 61.45 ± 3.34 mg GAE/g extract, respectively) (p < 0.05). In contrast, the EtOH extract showed the highest TFC (29.92 ± 3.42 mg QE/g extract) (p < 0.05). The EtOH and AqE extracts also demonstrated the strongest antioxidant activities in the DPPH (IC50: 307.07 ± 7.18 μg/mL and 316.86 ± 11.78 μg/mL), ABTS (IC50: 160.21 ± 5.43 μg/mL and 208.45 ± 3.84 μg/ mL) (p < 0.05), and FRAP (4.69 ± 0.08 and 4.96 ± 0.07 mM FeSO4/mg extract) assays. The antibacterial activity was observed exclusively against Gram-positive bacteria. Among all the extracts, the AqE extract at a concentration of 100 mg/mL exhibited significant antibacterial effects against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Bacillus cereus, with zones of inhibition measuring 15.43 ± 0.46 mm, 14.76 ± 0.58 mm, and 15.66 ± 1.04 mm, respectively. Conclusion: Ethanol-based extracts of C. hastata leaves demonstrate high antioxidant and antibacterial activities and represent a promising source of bioactive compounds for developing natural therapeutic agents.

10aAntibacterial10aAntioxidant10aCissus hastata10aLeaves10aPhytochemical1 aSingdam, Patcharin1 aKamnate, Anussara1 aSomsap, On-Anong1 aTohkayomatee, Ruhainee uhttps://phcogj.com/article/2530