02163nas a2200193 4500008004100000245012200041210006900163260001900232300001200251490000700263520154000270653001601810653001001826653000801836653003101844100003001875700002801905856003601933 2025 eng d00aIntegrated Phytochemical Evaluation of Triphala Extract: TLC Detection and GC–MS Elucidation of Bioactive Compounds0 aIntegrated Phytochemical Evaluation of Triphala Extract TLC Dete cSeptember 2025 a602-6070 v173 a

Triphala, a classical Ayurvedic polyherbal formulation composed of Terminalia chebula, Terminalia bellirica, and Phyllanthus emblica, is well known for its therapeutic efficacy and wide range of biological applications. Its pharmacological potential is attributed to a rich diversity of phytoconstituents, including alkaloids, flavonoids, tannins, and phenolic compounds. Scientific validation and standardization of such formulations require systematic phytochemical evaluation using both conventional and advanced analytical techniques. The present study was undertaken to investigate the phytochemicals composition of Triphala extract using TLC and GC-MS. TLC was done to detect gallic gallic acid a major component of Triphala. GCMS analysis was carried out enabling the separation and identification of volatile components based upon the retention time and mass fragmentation patterns. Several compounds of pharmacological relevance were identified including phenolic acids fatty acids and flavonoid derivatives which are consistent with the known antioxidant and therapeutic properties of Triphala. This integrated approach combining TLC and GC-MS along with standard biochemical testing provided both qualitative and confirmatory data on the phytoconstituents of Triphala. The fidings not only support the traditional claims but also contribute to a scientific validation ensuring quality and paving the way for further pharmaceutical and nutraceutical approaches.

10aGallic acid10aGC-MS10aTLC10aTriphala Phytoconstituents1 aRevankar, Hrithik, Satish1 aBhilegaonkar, Shilpa, P uhttps://phcogj.com/article/2545