02327nas a2200229 4500008004100000245014900041210006900190260001800259300001200277490000700289520158800296653001101884653001901895653001401914653001901928653002101947653002101968100001901989700002702008700002602035856003602061 2025 eng d00aHepatoprotective Potential of Coffea arabica Extract Against Carbon Tetrachloride Induced Liver Damage: Ultrasonographic and Cytokine Evaluation0 aHepatoprotective Potential of Coffea arabica Extract Against Car cDecember 2025 a742-7500 v173 a

Carbon tetrachloride (CCl₄) is a hepatotoxic agent widely used to model liver injury in experimental animals, and coffee (Coffea arabica) extract is known to contain various bioactive compounds with antioxidant and anti-inflammatory potential. This study aimed to evaluate the hepatoprotective activity of Coffea arabica extract in male Wistar rats induced with CCl₄ through phytochemical analysis, liver biochemical parameters (albumin, bilirubin, SGOT, SGPT), inflammatory cytokines (TNF-α, IL-6, CRP), and liver morphology observations using ultrasonography and histopathology. The extract contained alkaloids, flavonoids, triterpenoids, saponins, and glycosides, with total phenolic and flavonoid contents of 51.32 mg GAE/g and 1.25 mg QE/g extract, respectively, and moderate antioxidant activity (IC₅₀ = 124.36 ppm). Administration of the extract at 300 mg/kg BW significantly improved liver structure, reduced TNF-α (120.62 ± 21.78 pg/mL), IL-6 (68 ± 14.44 pg/mL), CRP (199.0 ± 41.5 pg/mL), SGOT (147.0 ± 34.28 g/dL), and SGPT (69.6 ± 9.94 g/dL) levels, while increasing albumin (2.94 ± 0.34 g/dL) and reducing bilirubin (0.09 ± 0.04 mg/dL), approaching normal conditions. The 300 mg/kg BW dose showed the most optimal protective effect compared to other treatment groups, as evidenced by improvements in biochemical parameters, liver morphology, and hepatic histology, indicating that Coffea arabica extract has strong potential as an effective hepatoprotective agent against CCl₄-induced liver damage

10aCCl₄10aCoffea arabica10aCytokines10aHepatotoxicity10aliver protection10aUltrasonographic1 aNovianti, Desi1 aLister, Nyoman, Ehrich1 aNasution, Ali, Napiah uhttps://phcogj.com/article/2564