02714nas a2200337 4500008004100000245011700041210006900158260001400227300001200241490000700253520162500260653003101885653002501916653002201941653002801963653002201991653002102013100002902034700002402063700002702087700002502114700002102139700002802160700002602188700002702214700003102241700002302272700002202295700002302317856003602340 2026 eng d00aTotal Phenolic and Flavonoid Contents, Antioxidant and Antiinflammatory Activities of Phellinus rimosus Extracts0 aTotal Phenolic and Flavonoid Contents Antioxidant and Antiinflam cJune 2026 a181-1860 v183 a

Background: Phellinus rimosus is a medicinal mushroom with potential bioactive properties, although scientific evidence supporting its pharmacological activities remains limited. Objective: To investigate the phenolic and flavonoid contents, antioxidant capacity, and anti-inflammatory activity of ethanolic and aqueous extracts of P. rimosus. Materials and Methods: Total phenolic content (TPC) and total flavonoid content (TFC) were determined using colorimetric assays. Antioxidant activity was evaluated by DPPH, ABTS, and FRAP methods. Anti-inflammatory activity was assessed by measuring nitric oxide (NO) inhibition in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Statistical analysis was performed at P < 0.05. Results: The ethanolic extract contained significantly higher TPC (361.04 ± 5.69 mg GAE/g extract) and TFC (646.55 ± 6.29 mg RE/g extract) than the aqueous extract. It exhibited strong antioxidant activity with IC₅₀ values of 9.56 ± 0.47 μg/mL (DPPH) and 5.40 ± 0.06 μg/mL (ABTS), and a FRAP value of 52.39 ± 0.25 mM Fe²⁺/100 mg extract. The ethanolic extract also significantly inhibited NO production (IC₅₀ = 54.40 ± 2.35 μg/mL) without cytotoxicity, whereas the aqueous extract showed no inhibitory effect. Conclusion: The ethanolic extract of P. rimosus demonstrates notable antioxidant and anti-inflammatory activities and may serve as a promising natural source of bioactive compounds.

10aAnti-inflammatory activity10aantioxidant activity10aFlavonoid content10anitric oxide inhibition10aPhellinus rimosus10aPhenolic content1 aLaovachirasuwan, Pornpun1 aThammavong, Sonesay1 aNaksuwankul, Khwanruan1 asuvanlasy, Sounantha1 aRittirod, Theera1 aPrasongchean, Weerapong1 aSangmahachai, Jidapha1 aChiangtong, Sutthipong1 aLerdpiriyasakulkit, Panwad1 aSriwong, Burin, T.1 aJatuten, Piyachet1 aPhadungkit, Methin uhttps://phcogj.com/article/2609